Structural highlights
Function
FLEN_PSEAE ATPase that plays an important role in maintaining flagellar number in Pseudomonas aeruginosa (PubMed:10629180, PubMed:28065505). Exhibits anti-activator activity against FleQ, the global transcriptional regulator of flagellar genes (PubMed:22581773, PubMed:28065505).[1] [2] [3]
Publication Abstract from PubMed
Diverse sigma factors associate with the RNA polymerase (RNAP) core enzyme to initiate transcription of specific target genes in bacteria. sigma(54)-Mediated transcription uses AAA+ activators that utilize their ATPase activity for transcription initiation. FleQ is a sigma(54)-dependent master transcriptional regulator of flagellar genes in Pseudomonas aeruginosa. The ATPase activity of FleQ is regulated via a P-loop ATPase, FleN, through protein-protein interaction. We report a high-resolution crystal structure of the AAA+ domain of FleQ in complex with antiactivator FleN. The data reveal that FleN allosterically prevents ATP binding to FleQ. Furthermore, FleN remodels the region of FleQ essential for engagement with sigma(54) for transcription initiation. Disruption of the conserved protein-protein interface, by mutation, shows motility and transcription defects in vivo and multiflagellate phenotype. Our study provides a detailed mechanism used by monoflagellate bacteria to fine-tune the expression of flagellar genes to form and maintain a single flagellum.
The antiactivator FleN uses an allosteric mechanism to regulate sigma(54)-dependent expression of flagellar genes in Pseudomonas aeruginosa.,Chanchal, Banerjee P, Raghav S, Goswami HN, Jain D Sci Adv. 2021 Oct 22;7(43):eabj1792. doi: 10.1126/sciadv.abj1792. Epub 2021 Oct , 20. PMID:34669473[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dasgupta N, Arora SK, Ramphal R. fleN, a gene that regulates flagellar number in Pseudomonas aeruginosa. J Bacteriol. 2000 Jan;182(2):357-64. PMID:10629180 doi:10.1128/JB.182.2.357-364.2000
- ↑ Baraquet C, Murakami K, Parsek MR, Harwood CS. The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP. Nucleic Acids Res. 2012 Aug;40(15):7207-18. PMID:22581773 doi:10.1093/nar/gks384
- ↑ Chanchal, Banerjee P, Jain D. ATP-Induced Structural Remodeling in the Antiactivator FleN Enables Formation of the Functional Dimeric Form. Structure. 2017 Feb 7;25(2):243-252. doi: 10.1016/j.str.2016.11.022. Epub 2017, Jan 5. PMID:28065505 doi:http://dx.doi.org/10.1016/j.str.2016.11.022
- ↑ Chanchal, Banerjee P, Raghav S, Goswami HN, Jain D. The antiactivator FleN uses an allosteric mechanism to regulate σ(54)-dependent expression of flagellar genes in Pseudomonas aeruginosa. Sci Adv. 2021 Oct 22;7(43):eabj1792. PMID:34669473 doi:10.1126/sciadv.abj1792
