Structural highlights
Function
PCHE_PSEAE Involved in the biosynthesis of the siderophore pyochelin (PubMed:9846750, PubMed:11208777). Accepts salicylate activated by PchD at the first peptidyl carrier domain (ArCP), and activates and fixes one molecule of cysteine at the second peptidyl carrier domain (PCP1) via a thioester linkage to the phosphopanthetheine moiety (By similarity). Then catalyzes the condensation reaction between the salicylate bound to the first site and the cysteine bound to the second site, and the cyclization of the cysteine to form the salicyl-thiazolinyl-S-PCP1 intermediate at the second site (By similarity). When this intermediate is released by the action of a thioesterase, it produces the antifungal antibiotic dihydroaeruginoic acid (Dha or hydroxyphenyl-thiazolinyl-carboxylate) (PubMed:11208777).[UniProtKB:A0A0H2ZGB9][1] [2]
References
- ↑ Reimmann C, Patel HM, Serino L, Barone M, Walsh CT, Haas D. Essential PchG-dependent reduction in pyochelin biosynthesis of Pseudomonas aeruginosa. J Bacteriol. 2001 Feb;183(3):813-20. PMID:11208777 doi:10.1128/JB.183.3.813-820.2001
- ↑ Reimmann C, Serino L, Beyeler M, Haa D. Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa. Microbiology (Reading). 1998 Nov;144 ( Pt 11):3135-3148. PMID:9846750 doi:10.1099/00221287-144-11-3135
