7l7w
From Proteopedia
Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E mutant
Structural highlights
FunctionDRL42_ARATH Probable disease resistance protein. Publication Abstract from PubMedPlant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis, a subfamily of "helper" NLRs are required by many "sensor" NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta and conferred cytoplasmic Ca(2+) influx in plant and human cells. NRG1.1-dependent Ca(2+) influx and cell death were sensitive to Ca(2+) channel blockers and were suppressed by mutations impacting oligomerization or plasma membrane enrichment. Ca(2+) influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another "helper" NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrate that Arabidopsis "helper" NLRs form Ca(2+)-permeable cation channels to directly regulate cytoplasmic Ca(2+) levels and consequent cell death. Thus, "helper" NLRs transduce cell death signals directly. Plant "helper" immune receptors are Ca(2+)-permeable nonselective cation channels.,Jacob P, Kim NH, Wu F, El-Kasmi F, Chi Y, Walton WG, Furzer OJ, Lietzan AD, Sunil S, Kempthorn K, Redinbo MR, Pei ZM, Wan L, Dangl JL Science. 2021 Jun 17. pii: science.abg7917. doi: 10.1126/science.abg7917. PMID:34140391[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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