| Structural highlights
Function
PP150_HCMVA Participates in the last steps of viral maturation and release. Associates with nuclear capsids prior to DNA encapsidation and later preserves the integrity of nucleocapsids through secondary envelopment at the assembly compartment. Interacts with host CCNA2 and thereby blocks the onset of lytic gene expression to promote establishment of a quiescent state of infection in undifferentiated cells.[1] [2] [3] [4] [5]
Publication Abstract from PubMed
Under the Baltimore nucleic acid-based virus classification scheme, the herpesvirus human cytomegalovirus (HCMV) is a Class I virus, meaning that it contains a double-stranded DNA genome-and no RNA. Here, we report sub-particle cryoEM reconstructions of HCMV virions at 2.9 A resolution revealing structures resembling non-coding transfer RNAs (tRNAs) associated with the virion's capsid-bound tegument protein, pp150. Through deep sequencing, we show that these RNA sequences match human tRNAs, and we built atomic models using the most abundant tRNA species. Based on our models, tRNA recruitment is mediated by the electrostatic interactions between tRNA phosphate groups and the helix-loop-helix motif of HCMV pp150. The specificity of these interactions may explain the absence of such tRNA densities in murine cytomegalovirus and other human herpesviruses.
Structure of human cytomegalovirus virion reveals host tRNA binding to capsid-associated tegument protein pp150.,Liu YT, Strugatsky D, Liu W, Zhou ZH Nat Commun. 2021 Sep 17;12(1):5513. doi: 10.1038/s41467-021-25791-1. PMID:34535641[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ AuCoin DP, Smith GB, Meiering CD, Mocarski ES. Betaherpesvirus-conserved cytomegalovirus tegument protein ppUL32 (pp150) controls cytoplasmic events during virion maturation. J Virol. 2006 Aug;80(16):8199-210. PMID:16873276 doi:http://dx.doi.org/10.1128/JVI.00457-06
- ↑ Tandon R, Mocarski ES. Control of cytoplasmic maturation events by cytomegalovirus tegument protein pp150. J Virol. 2008 Oct;82(19):9433-44. doi: 10.1128/JVI.00533-08. Epub 2008 Jul 23. PMID:18653449 doi:http://dx.doi.org/10.1128/JVI.00533-08
- ↑ Indran SV, Britt WJ. A role for the small GTPase Rab6 in assembly of human cytomegalovirus. J Virol. 2011 May;85(10):5213-9. doi: 10.1128/JVI.02605-10. Epub 2011 Mar 16. PMID:21411515 doi:http://dx.doi.org/10.1128/JVI.02605-10
- ↑ Dai X, Yu X, Gong H, Jiang X, Abenes G, Liu H, Shivakoti S, Britt WJ, Zhu H, Liu F, Zhou ZH. The smallest capsid protein mediates binding of the essential tegument protein pp150 to stabilize DNA-containing capsids in human cytomegalovirus. PLoS Pathog. 2013 Aug;9(8):e1003525. doi: 10.1371/journal.ppat.1003525. Epub 2013, Aug 15. PMID:23966856 doi:http://dx.doi.org/10.1371/journal.ppat.1003525
- ↑ Bogdanow B, Weisbach H, von Einem J, Straschewski S, Voigt S, Winkler M, Hagemeier C, Wiebusch L. Human cytomegalovirus tegument protein pp150 acts as a cyclin A2-CDK-dependent sensor of the host cell cycle and differentiation state. Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17510-5. doi:, 10.1073/pnas.1312235110. Epub 2013 Oct 7. PMID:24101496 doi:http://dx.doi.org/10.1073/pnas.1312235110
- ↑ Liu YT, Strugatsky D, Liu W, Zhou ZH. Structure of human cytomegalovirus virion reveals host tRNA binding to capsid-associated tegument protein pp150. Nat Commun. 2021 Sep 17;12(1):5513. PMID:34535641 doi:10.1038/s41467-021-25791-1
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