7n9i

From Proteopedia

1.4A Structure of Drosophila melanogaster Frataxin

Structural highlights

7n9i is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FRDA_DROME Promotes the biosynthesis of heme as well as the assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways (PubMed:18540637). May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+) (PubMed:18540637). May be able to store large amounts of the metal in the form of a ferrihydrite mineral by oligomerization (PubMed:18540637). Required for ecdysteroidogenesis in the prothoracic gland which is necessary for larval to pupal transition (PubMed:25628335).^[1] ^[2]

Publication Abstract from PubMed

Friedreich's ataxia (FRDA) is a hereditary cardiodegenerative and neurodegenerative disease that affects 1 in 50 000 Americans. FRDA arises from either a cellular inability to produce sufficient quantities or the production of a nonfunctional form of the protein frataxin, a key molecule associated with mitochondrial iron-sulfur cluster biosynthesis. Within the mitochondrial iron-sulfur cluster (ISC) assembly pathway, frataxin serves as an allosteric regulator for cysteine desulfurase, the enzyme that provides sulfur for [2Fe-2S] cluster assembly. Frataxin is a known iron-binding protein and is also linked to the delivery of ferrous ions to the scaffold protein, the ISC molecule responsible for the direct assembly of [2Fe-2S] clusters. The goal of this report is to provide structural details of the Drosophila melanogaster frataxin ortholog (Dfh), using both X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, in order to provide the foundational insight needed to understand the structure-function correlation of the protein. Additionally, NMR iron(II) titrations were used to provide metal contacts on the protein to better understand how it binds iron and aids its delivery to the ISC scaffold protein. Here, the structural and functional similarities of Dfh to its orthologs are also outlined. Structural data show that bacterial, yeast, human and Drosophila frataxins are structurally similar, apart from a structured C-terminus in Dfh that is likely to aid in protein stability. The iron-binding location on helix 1 and strand 1 of Dfh is also conserved across orthologs.

Drosophila melanogaster frataxin: protein crystal and predicted solution structure with identification of the iron-binding regions.,Rodrigues AV, Batelu S, Hinton TV, Rotondo J, Thompson L, Brunzelle JS, Stemmler TL Acta Crystallogr D Struct Biol. 2023 Jan 1;79(Pt 1):22-30. doi: , 10.1107/S2059798322011639. Epub 2023 Jan 1. PMID:36601804^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kondapalli KC, Kok NM, Dancis A, Stemmler TL. Drosophila frataxin: an iron chaperone during cellular Fe-S cluster bioassembly. Biochemistry. 2008 Jul 1;47(26):6917-27. doi: 10.1021/bi800366d. Epub 2008 Jun , 10. PMID:18540637 doi:http://dx.doi.org/10.1021/bi800366d
↑ Palandri A, L'hôte D, Cohen-Tannoudji J, Tricoire H, Monnier V. Frataxin inactivation leads to steroid deficiency in flies and human ovarian cells. Hum Mol Genet. 2015 May 1;24(9):2615-26. PMID:25628335 doi:10.1093/hmg/ddv024
↑ Rodrigues AV, Batelu S, Hinton TV, Rotondo J, Thompson L, Brunzelle JS, Stemmler TL. Drosophila melanogaster frataxin: protein crystal and predicted solution structure with identification of the iron-binding regions. Acta Crystallogr D Struct Biol. 2023 Jan 1;79(Pt 1):22-30. doi: , 10.1107/S2059798322011639. Epub 2023 Jan 1. PMID:36601804 doi:http://dx.doi.org/10.1107/S2059798322011639