7nhf
From Proteopedia
Crystal structure of Arabidopsis thaliana Pdx1K166R
Structural highlights
FunctionPDX13_ARATH Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by PDX2. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively. Also plays an indirect role in resistance to singlet oxygen-generating photosensitizers.[1] [2] [3] Publication Abstract from PubMedThe Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis. Trapping and structural characterisation of a covalent intermediate in vitamin B6 biosynthesis catalysed by the Pdx1 PLP synthase.,Rodrigues MJ, Giri N, Royant A, Zhang Y, Bolton R, Evans G, Ealick SE, Begley T, Tews I RSC Chem Biol. 2021 Oct 25;3(2):227-230. doi: 10.1039/d1cb00160d. eCollection, 2022 Feb 9. PMID:35360887[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Arabidopsis thaliana | Large Structures | Begley T | Bolton R | Ealick SE | Evans G | Giri N | Rodrigues MJ | Royant A | Tews I | Zhang Y
