7ogp

Publication Abstract from PubMed

Bacteriophage PhiKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, intrinsically antibiotic resistant, pathogen that kills tens of thousands worldwide each year. PhiKZ is an incredibly interesting virus, expressing many systems that the host already possesses. On infection, it forms a 'nucleus', erecting a barrier around its genome to exclude host endonucleases and CRISPR-Cas systems. PhiKZ infection is independent of the host transcriptional apparatus. It expresses two different multi-subunit RNA polymerases (RNAPs): the virion RNAP (vRNAP) is injected with the viral DNA during infection to transcribe early genes, including those encoding the non-virion RNAP (nvRNAP), which transcribes all further genes. PhiKZ nvRNAP is formed by four polypeptides thought to represent homologues of the eubacterial beta/beta' subunits, and a fifth with unclear homology, but essential for transcription. We have resolved the structure of PhiKZ nvRNAP to better than 3.0 A, shedding light on its assembly, homology, and the biological role of the fifth subunit: it is an embedded, integral member of the complex, the position, structural homology and biochemical role of which imply that it has evolved from an ancestral homologue to sigma-factor.

Structure of the bacteriophage PhiKZ non-virion RNA polymerase.,deYMartin Garrido N, Orekhova M, Lai Wan Loong YTE, Litvinova A, Ramlaul K, Artamonova T, Melnikov AS, Serdobintsev P, Aylett CHS, Yakunina M Nucleic Acids Res. 2021 Jul 21;49(13):7732-7739. doi: 10.1093/nar/gkab539. PMID:34181731^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.