7oie
From Proteopedia
Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 5B
Structural highlights
DiseaseRM03_HUMAN Combined oxidative phosphorylation defect type 9. The disease is caused by mutations affecting the gene represented in this entry. FunctionPublication Abstract from PubMedAssembly of the mitoribosome is largely enigmatic and involves numerous assembly factors. Little is known about their function and the architectural transitions of the pre-ribosomal intermediates. Here, we solve cryo-EM structures of the human 39S large subunit pre-ribosomes, representing five distinct late states. Besides the MALSU1 complex used as bait for affinity purification, we identify several assembly factors, including the DDX28 helicase, MRM3, GTPBP10 and the NSUN4-mTERF4 complex, all of which keep the 16S rRNA in immature conformations. The late transitions mainly involve rRNA domains IV and V, which form the central protuberance, the intersubunit side and the peptidyltransferase center of the 39S subunit. Unexpectedly, we find deacylated tRNA in the ribosomal E-site, suggesting a role in 39S assembly. Taken together, our study provides an architectural inventory of the distinct late assembly phase of the human 39S mitoribosome. A distinct assembly pathway of the human 39S late pre-mitoribosome.,Cheng J, Berninghausen O, Beckmann R Nat Commun. 2021 Jul 27;12(1):4544. doi: 10.1038/s41467-021-24818-x. PMID:34315873[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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