7oz4

Publication Abstract from PubMed

The varphiRSA1 bacteriophage has been isolated from Ralstonia solanacearum, a gram negative bacteria having a significant economic impact on many important crops. We solved the three-dimensional structure of the varphiRSA1 mature capsid to 3.9 A resolution by cryo-electron microscopy. The capsid shell, that contains the 39 kbp of dsDNA genome, has an icosahedral symmetry characterized by an unusual triangulation number of T = 7, dextro. The varphiRSA1 capsid is composed solely of the polymerization of the major capsid protein, gp8, which exhibits the typical "Johnson" fold first characterized in E. coli bacteriophage HK97. As opposed to the latter, the varphiRSA1 mature capsid is not stabilized by covalent crosslinking between its subunits, nor by the addition of a decoration protein. We further describe the molecular interactions occurring between the subunits of the varphiRSA1 capsid and their relationships with the other known bacteriophages.

High Resolution Structure of the Mature Capsid of Ralstonia solanacearum Bacteriophage varphiRSA1 by Cryo-Electron Microscopy.,Effantin G, Fujiwara A, Kawasaki T, Yamada T, Schoehn G Int J Mol Sci. 2021 Oct 13;22(20). pii: ijms222011053. doi:, 10.3390/ijms222011053. PMID:34681713^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.