7pli

Structural highlights

Function

DBPA_ECOLI DEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes. Requires a single-stranded RNA loading site on the 3' side of the substrate helix.[HAMAP-Rule:MF_00965]^{[1] [2] [3] [4] [5] [6] [7] [8] [9]}

See Also

• Helicase 3D structures

References

1. ↑ Tsu CA, Kossen K, Uhlenbeck OC. The Escherichia coli DEAD protein DbpA recognizes a small RNA hairpin in 23S rRNA. RNA. 2001 May;7(5):702-9. doi: 10.1017/s1355838201010135. PMID:11350034 doi:http://dx.doi.org/10.1017/s1355838201010135
2. ↑ Diges CM, Uhlenbeck OC. Escherichia coli DbpA is an RNA helicase that requires hairpin 92 of 23S rRNA. EMBO J. 2001 Oct 1;20(19):5503-12. doi: 10.1093/emboj/20.19.5503. PMID:11574482 doi:http://dx.doi.org/10.1093/emboj/20.19.5503
3. ↑ Diges CM, Uhlenbeck OC. Escherichia coli DbpA is a 3' --> 5' RNA helicase. Biochemistry. 2005 May 31;44(21):7903-11. doi: 10.1021/bi050033x. PMID:15910005 doi:http://dx.doi.org/10.1021/bi050033x
4. ↑ Henn A, Cao W, Hackney DD, De La Cruz EM. The ATPase cycle mechanism of the DEAD-box rRNA helicase, DbpA. J Mol Biol. 2008 Mar 14;377(1):193-205. doi: 10.1016/j.jmb.2007.12.046. Epub 2007, Dec 28. PMID:18237742 doi:http://dx.doi.org/10.1016/j.jmb.2007.12.046
5. ↑ Sharpe Elles LM, Sykes MT, Williamson JR, Uhlenbeck OC. A dominant negative mutant of the E. coli RNA helicase DbpA blocks assembly of the 50S ribosomal subunit. Nucleic Acids Res. 2009 Oct;37(19):6503-14. doi: 10.1093/nar/gkp711. Epub 2009, Sep 4. PMID:19734347 doi:http://dx.doi.org/10.1093/nar/gkp711
6. ↑ Henn A, Cao W, Licciardello N, Heitkamp SE, Hackney DD, De La Cruz EM. Pathway of ATP utilization and duplex rRNA unwinding by the DEAD-box helicase, DbpA. Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4046-50. doi:, 10.1073/pnas.0913081107. Epub 2010 Feb 16. PMID:20160110 doi:http://dx.doi.org/10.1073/pnas.0913081107
7. ↑ Fuller-Pace FV, Nicol SM, Reid AD, Lane DP. DbpA: a DEAD box protein specifically activated by 23s rRNA. EMBO J. 1993 Sep;12(9):3619-26. PMID:8253085
8. ↑ Boddeker N, Stade K, Franceschi F. Characterization of DbpA, an Escherichia coli DEAD box protein with ATP independent RNA unwinding activity. Nucleic Acids Res. 1997 Feb 1;25(3):537-45. doi: 10.1093/nar/25.3.537. PMID:9016593 doi:http://dx.doi.org/10.1093/nar/25.3.537
9. ↑ Tsu CA, Uhlenbeck OC. Kinetic analysis of the RNA-dependent adenosinetriphosphatase activity of DbpA, an Escherichia coli DEAD protein specific for 23S ribosomal RNA. Biochemistry. 1998 Dec 1;37(48):16989-96. doi: 10.1021/bi981837y. PMID:9836593 doi:http://dx.doi.org/10.1021/bi981837y