7un6

Structural highlights

Function

UBE2O_HUMAN E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).^{[1] [2] [3] [4]}

References

1. ↑ Zhang X, Zhang J, Zhang L, van Dam H, ten Dijke P. UBE2O negatively regulates TRAF6-mediated NF-kappaB activation by inhibiting TRAF6 polyubiquitination. Cell Res. 2013 Mar;23(3):366-77. doi: 10.1038/cr.2013.21. Epub 2013 Feb 5. PMID:23381138 doi:http://dx.doi.org/10.1038/cr.2013.21
2. ↑ Hao YH, Doyle JM, Ramanathan S, Gomez TS, Jia D, Xu M, Chen ZJ, Billadeau DD, Rosen MK, Potts PR. Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination. Cell. 2013 Feb 28;152(5):1051-64. doi: 10.1016/j.cell.2013.01.051. PMID:23452853 doi:http://dx.doi.org/10.1016/j.cell.2013.01.051
3. ↑ Zhang X, Zhang J, Bauer A, Zhang L, Selinger DW, Lu CX, Ten Dijke P. Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6. EMBO J. 2013 Apr 3;32(7):996-1007. doi: 10.1038/emboj.2013.38. Epub 2013 Mar 1. PMID:23455153 doi:http://dx.doi.org/10.1038/emboj.2013.38
4. ↑ Mashtalir N, Daou S, Barbour H, Sen NN, Gagnon J, Hammond-Martel I, Dar HH, Therrien M, Affar el B. Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O. Mol Cell. 2014 May 8;54(3):392-406. doi: 10.1016/j.molcel.2014.03.002. Epub 2014 , Apr 3. PMID:24703950 doi:http://dx.doi.org/10.1016/j.molcel.2014.03.002