Structural highlights
7v0d is a 2 chain structure with sequence from Kitasatospora aureofaciens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 2.6Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
CTCP_KITAU Involved in the biosynthesis of chlorotetracycline (CTC), an important member from antibiotics tetracycline (TC) family, which inhibits protein synthesis in bacteria and is widely involved in clinical therapy, animal feeds and aquaculture. Utilizes FADH(2) supplied by the flavin reductase CtcQ, to catalyze the chlorination of tetracycline (TC) at C7 position, leading to the production of 7-chlorotetracycline. The enzyme forms a lysine chloramine intermediate on an internal lysine residue before transferring the chlorine to the substrate. It is stereo-selective for the 4S (natural) isomer of tetracycline.[1]
References
- ↑ Zhu T, Cheng X, Liu Y, Deng Z, You D. Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens. Metab Eng. 2013 Sep;19:69-78. doi: 10.1016/j.ymben.2013.06.003. Epub 2013 Jun 22. PMID:23800859 doi:http://dx.doi.org/10.1016/j.ymben.2013.06.003
