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Publication Abstract from PubMed

Fluorinated nucleotides are invaluable for (19) F NMR studies of nucleic acid structure and function. Here, we synthesized 4'-SCF3 -thymidine (T 4 ' - SCF 3 ${{^{4{^\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ ) and incorporated it into DNA by means of solid-phase DNA synthesis. NMR studies showed that the 4'-SCF3 group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated by various higher order DNA structures. The three magnetically equivalent fluorine atoms in 4'-SCF3 -DNA constitute an isolated spin system, offering high (19) F NMR sensitivity and excellent resolution of the positioning of T 4 ' - SCF 3 ${{^{4{^\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ within various secondary and tertiary DNA structures. The high structural adaptability and high sensitivity of T 4 ' - SCF 3 ${{^{4{^\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ make it a valuable (19) F NMR probe for quantitatively distinguishing diverse DNA structures with single-nucleotide resolution and for monitoring the dynamics of interactions in the minor groove of double-stranded DNA.

4'-SCF3 -Labeling Constitutes a Sensitive (19) F NMR Probe for Characterization of Interactions in the Minor Groove of DNA.,Li Q, Trajkovski M, Fan C, Chen J, Zhou Y, Lu K, Li H, Su X, Xi Z, Plavec J, Zhou C Angew Chem Int Ed Engl. 2022 Nov 21;61(47):e202201848. doi:, 10.1002/anie.202201848. Epub 2022 Oct 19. PMID:36163470^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.