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Publication Abstract from PubMed

Inhibiting alpha-glucosidase activity is important in controlling postprandial hyperglycemia and, thus, helping to manage type-2 diabetes mellitus (T2DM). In the present study, we purified a hypothetical protein of carrots called DCHP (Daucus Carrot hypoglycemic peptide), and their inhibitory effects on alpha-glucosidase, as well as related mechanisms, were investigated. The recombinant DCHP protein with a molecular weight of 8 kDa showed strong inhibitory activity against alpha-glycosidase and maintained good stability in solution. DCHP exhibited no inhibitory activity but was tolerant to trypsin and chymotrypsin. Cellular experiments demonstrated that glucose consumption and lactic acid production increased rapidly when treated with DCHP in Caco-2 and HepG2 cells. DCHP crystal was generated, and the crystal structure, which was similar to that of rBTI and consisted of a central alpha-helix and a two-stranded beta-sheet with a unique loop region. The interaction between DCHP and alpha-glycosidase was investigated by molecular docking and site-directed mutation, which revealed that Glu43, Pro46, Thr47 Thr48 and Gln49 are the key residues in DCHP that inhibit alpha-glycosidase activity. This work provides potential bioactive peptides as functional foods or nutraceutical supplements in preventing and managing T2DM.

Characterization of a thermostable, protease-tolerant inhibitor of alpha-glycosidase from carrot: A potential oral additive for treatment of diabetes.,Hao Y, Guo T, Ren J, Wang Y, Wang L, Shi Y, Feng W Int J Biol Macromol. 2022 Jun 1;209(Pt A):1271-1279. doi:, 10.1016/j.ijbiomac.2022.04.110. Epub 2022 Apr 20. PMID:35460754^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.