7ypb

From Proteopedia

Cryo-EM structure of Escherichia coli release complex of transcription termination (TTC-release)

Structural highlights

7ypb is a 9 chain structure with sequence from Escherichia coli K-12 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.48Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPOA_ECOLI DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059]

Publication Abstract from PubMed

Efficient and accurate termination is required for gene transcription in all living organisms(1,2). Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription through a factor-independent termination pathway(3,4)-called intrinsic termination transcription in bacteria-in which RNA polymerase recognizes terminator sequences, stops nucleotide addition and releases nascent RNA spontaneously. Here we report a set of single-particle cryo-electron microscopy structures of Escherichia coli transcription intrinsic termination complexes representing key intermediate states of the event. The structures show how RNA polymerase pauses at terminator sequences, how the terminator RNA hairpin folds inside RNA polymerase, and how RNA polymerase rewinds the transcription bubble to release RNA and then DNA. These macromolecular snapshots define a structural mechanism for bacterial intrinsic termination and a pathway for RNA release and DNA collapse that is relevant for factor-independent termination by all RNA polymerases.

Structural basis for intrinsic transcription termination.,You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y Nature. 2023 Jan;613(7945):783-789. doi: 10.1038/s41586-022-05604-1. Epub 2023 , Jan 11. PMID:36631609^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y. Structural basis for intrinsic transcription termination. Nature. 2023 Jan;613(7945):783-789. PMID:36631609 doi:10.1038/s41586-022-05604-1