Structural highlights
8aq8 is a 4 chain structure with sequence from Stenotrophomonas maltophilia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.95Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
The resistance of the emerging human pathogen Stenotrophomonas maltophilia to tetracycline antibiotics mainly depends on multidrug efflux pumps and ribosomal protection enzymes. However, the genomes of several strains of this Gram-negative bacterium code for a FAD-dependent monooxygenase (SmTetX) homologous to tetracycline destructases. This protein was recombinantly produced and its structure and function were investigated. Activity assays using SmTetX showed its ability to modify oxytetracycline with a catalytic rate comparable to those of other destructases. SmTetX shares its fold with the tetracycline destructase TetX from Bacteroides thetaiotaomicron; however, its active site possesses an aromatic region that is unique in this enzyme family. A docking study confirmed tetracycline and its analogues to be the preferred binders amongst various classes of antibiotics.
Tetracycline-modifying enzyme SmTetX from Stenotrophomonas maltophilia.,Maly M, Kolenko P, Stransky J, Svecova L, Duskova J, Koval' T, Skalova T, Trundova M, Adamkova K, Cerny J, Bozikova P, Dohnalek J Acta Crystallogr F Struct Biol Commun. 2023 Jul 1;79(Pt 7):180-192. doi: , 10.1107/S2053230X23005381. Epub 2023 Jul 5. PMID:37405486[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Malý M, Kolenko P, Stránský J, Švecová L, Dušková J, Koval' T, Skálová T, Trundová M, Adámková K, Černý J, Božíková P, Dohnálek J. Tetracycline-modifying enzyme SmTetX from Stenotrophomonas maltophilia. Acta Crystallogr F Struct Biol Commun. 2023 Jul 1;79(Pt 7):180-192. PMID:37405486 doi:10.1107/S2053230X23005381
