Structural highlights
8cff is a 8 chain structure with sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.57Å |
| Ligands: | , , , , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
AIOA_ALCFA Involved in the detoxification of arsenic. Oxidizes As(III)O3(3-) (arsenite) to the somewhat less toxic As(V)O4(3-) (arsenate).
Publication Abstract from PubMed
Arsenic contamination of groundwater is among one of the biggest health threats affecting millions of people in the world. There is an urgent need for efficient arsenic biosensors where the use of arsenic metabolizing enzymes can be explored. In this work, we have solved four crystal structures of arsenite oxidase (Aio) in complex with arsenic and antimony oxyanions and the structures determined correspond to intermediate states of the enzymatic mechanism. These structural data were complemented with density-functional theory calculations providing a unique view of the molybdenum active site at different time points that, together with mutagenesis data, enabled to clarify the enzymatic mechanism and the molecular determinants for the oxidation of As(III) to the less toxic As(V) species.
Arsenite oxidase in complex with antimonite and arsenite oxyanions: Insights into the catalytic mechanism.,Engrola F, Correia MAS, Watson C, Romao CC, Veiros LF, Romao MJ, Santos-Silva T, Santini JM J Biol Chem. 2023 Aug;299(8):105036. doi: 10.1016/j.jbc.2023.105036. Epub 2023 , Jul 11. PMID:37442232[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Engrola F, Correia MAS, Watson C, Romão CC, Veiros LF, Romão MJ, Santos-Silva T, Santini JM. Arsenite oxidase in complex with antimonite and arsenite oxyanions: Insights into the catalytic mechanism. J Biol Chem. 2023 Aug;299(8):105036. PMID:37442232 doi:10.1016/j.jbc.2023.105036
