8cs1

Publication Abstract from PubMed

<p class="Text" style="margin: 0in 0in 8pt; text-align: justify; line-height: 24px; font-size: 12pt; font-family: "Times New Roman", serif; color: rgb(0, 0, 0);">Non-canonical interactions in DNA remain under-explored in DNA nanotechnology. Recently, many structures with non-canonical motifs have been discovered, notably a hexagonal arrangement of typically rhombohedral DNA tensegrity triangles that forms through non-canonical sticky end interactions. Here, we find a series of mechanisms to program a hexagonal arrangement using: the sticky end sequence; triangle edge torsional stress; and crystallization condition. We showcase cross-talking between Watson-Crick and non-canonical sticky ends in which the ratio between the two dictates segregation by crystal forms or combination into composite crystals. Finally, we develop a method for reconfiguring the long-range geometry of formed crystals from rhombohedral to hexagonal and <italic>vice versa</italic>. These data demonstrate fine control over non-canonical motifs and their topological self-assembly. This will vastly increase the programmability, functionality, and versatility of rationally designed DNA constructs.<o:p></o:p></p>.

Programmable 3D Hexagonal Geometry of DNA Tensegrity Triangles.,Lu B, Woloszyn K, Ohayon YP, Yang B, Zhang C, Mao C, Seeman NC, Vecchioni S, Sha R Angew Chem Int Ed Engl. 2022 Dec 15. doi: 10.1002/anie.202213451. PMID:36520622^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.