8dqw

Structural highlights

Function

RAD17_YEAST Component of the checkpoint clamp complex involved in the surveillance mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Associates with sites of DNA damage and modulates the MEC1 signaling pathway and the activation of RAD53 in response to DNA damage at phase G1. The complex also physically regulates DNA polymerase zeta-dependent mutagenesis by controlling the access of polymerase zeta to damaged DNA. Contrary to its human counterpart, the 9-1-1 complex, the checkpoint clamp complex shows no detectable exonuclease activity.^{[1] [2] [3] [4] [5] [6] [7] [8]}

References

1. ↑ Giannattasio M, Sommariva E, Vercillo R, Lippi-Boncambi F, Liberi G, Foiani M, Plevani P, Muzi-Falconi M. A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12997-3002. PMID:12271137 doi:10.1073/pnas.202463999
2. ↑ Majka J, Burgers PM. Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2249-54. Epub 2003 Feb 25. PMID:12604797 doi:http://dx.doi.org/10.1073/pnas.0437148100
3. ↑ Giannattasio M, Sabbioneda S, Minuzzo M, Plevani P, Muzi-Falconi M. Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1. J Biol Chem. 2003 Jun 20;278(25):22303-8. PMID:12672803 doi:10.1074/jbc.M301260200
4. ↑ Majka J, Burgers PM. Function of Rad17/Mec3/Ddc1 and its partial complexes in the DNA damage checkpoint. DNA Repair (Amst). 2005 Sep 28;4(10):1189-94. PMID:16137930 doi:10.1016/j.dnarep.2005.07.008
5. ↑ Sabbioneda S, Minesinger BK, Giannattasio M, Plevani P, Muzi-Falconi M, Jinks-Robertson S. The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. J Biol Chem. 2005 Nov 18;280(46):38657-65. PMID:16169844 doi:10.1074/jbc.M507638200
6. ↑ Lydall D, Weinert T. Yeast checkpoint genes in DNA damage processing: implications for repair and arrest. Science. 1995 Dec 1;270(5241):1488-91. PMID:7491494 doi:10.1126/science.270.5241.1488
7. ↑ Siede W, Nusspaumer G, Portillo V, Rodriguez R, Friedberg EC. Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae. Nucleic Acids Res. 1996 May 1;24(9):1669-75. PMID:8649984 doi:10.1093/nar/24.9.1669
8. ↑ Kondo T, Matsumoto K, Sugimoto K. Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway. Mol Cell Biol. 1999 Feb;19(2):1136-43. PMID:9891048 doi:10.1128/MCB.19.2.1136