8gu3

Structural highlights

Function

MET16_CAEEL RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating splicing of S-adenosylmethionine synthase transcripts (sams-3, sams-4 and sams-5) (PubMed:33930289). Able to N6-methylate a subset of mRNAs containing the 5'UACAGAAAC-3' nonamer sequence (PubMed:33930289). Plays a key role in S-adenosyl-L-methionine homeostasis: under rich-diet conditions, catalyzes N6-methylation of S-adenosylmethionine synthase mRNAs (sams-3, sams-4 and sams-5), directly inhibiting splicing and protein production of S-adenosylmethionine synthase (PubMed:33930289). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA) (PubMed:33930289). Required for gamete production, inhibiting germ cell proliferative fate and ensuring germ cell meiotic development (PubMed:19596901, PubMed:19752194). Also promotes progression of the mitotic cell cycle in those germ cells that continue to proliferate (PubMed:19596901, PubMed:19752194). Plays a role in the development of the vulva, somatic gonad and embryo (PubMed:19596901).^{[1] [2] [3]}

References

1. ↑ Dorsett M, Westlund B, Schedl T. METT-10, a putative methyltransferase, inhibits germ cell proliferative fate in Caenorhabditis elegans. Genetics. 2009 Sep;183(1):233-47. doi: 10.1534/genetics.109.105270. Epub 2009 Jul , 13. PMID:19596901 doi:http://dx.doi.org/10.1534/genetics.109.105270
2. ↑ Dorsett M, Schedl T. A role for dynein in the inhibition of germ cell proliferative fate. Mol Cell Biol. 2009 Nov;29(22):6128-39. doi: 10.1128/MCB.00815-09. Epub 2009 Sep , 14. PMID:19752194 doi:http://dx.doi.org/10.1128/MCB.00815-09
3. ↑ Mendel M, Delaney K, Pandey RR, Chen KM, Wenda JM, Vagbo CB, Steiner FA, Homolka D, Pillai RS. Splice site m(6)A methylation prevents binding of U2AF35 to inhibit RNA splicing. Cell. 2021 Jun 10;184(12):3125-3142.e25. doi: 10.1016/j.cell.2021.03.062. Epub , 2021 Apr 29. PMID:33930289 doi:http://dx.doi.org/10.1016/j.cell.2021.03.062