8i76
From Proteopedia
Crystal structure of decarboxylated osteocalcin at pH 2.0 without glycerol
Structural highlights
FunctionOSTCN_BOVIN Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Publication Abstract from PubMedAn acidic environment in bone is essential for bone metabolism and the production of decarboxylated osteocalcin, which functions as a regulatory hormone of glucose metabolism. Here, we describe the high-resolution X-ray crystal structure of decarboxylated osteocalcin under acidic conditions. Decarboxylated osteocalcin at pH 2.0 retains the alpha-helix structure of native osteocalcin with three gamma-carboxyglutamic acid residues at neutral pH. This implies that decarboxylated osteocalcin is stable under an acidic environment in bone. In addition, site-directed mutagenesis revealed that Glu17 and Glu21 are important for the adiponectin-inducing activity of decarboxylated osteocalcin. These findings suggest that the receptor of decarboxylated osteocalcin responds to the negative charge in helix 1 of osteocalcin. Structural and mutational analyses of decarboxylated osteocalcin provide insight into its adiponectin-inducing activity.,Mizuguchi M, Yokoyama T, Otani T, Kuribara S, Nabeshima Y, Obita T, Hirata M, Kawano K FEBS Lett. 2023 Jun;597(11):1479-1488. doi: 10.1002/1873-3468.14618. Epub 2023 , Apr 4. PMID:36976525[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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