| Structural highlights
Function
DX39A_HUMAN Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.[1] [2]
Publication Abstract from PubMed
mRNA export is an essential pathway for the regulation of gene expression. In humans, closely related RNA helicases, UAP56 and URH49, shape selective mRNA export pathways through the formation of distinct complexes, known as apo-TREX and apo-AREX complexes, and their subsequent remodeling into similar ATP-bound complexes. Therefore, defining the unidentified components of the apo-AREX complex and elucidating the molecular mechanisms underlying the formation of distinct apo-complexes is key to understanding their functional divergence. In this study, we identify additional apo-AREX components physically and functionally associated with URH49. Furthermore, by comparing the structures of UAP56 and URH49 and performing an integrated analysis of their chimeric mutants, we exhibit unique structural features that would contribute to the formation of their respective complexes. This study provides insights into the specific structural and functional diversification of these two helicases that diverged from the common ancestral gene Sub2.
Structural differences between the closely related RNA helicases, UAP56 and URH49, fashion distinct functional apo-complexes.,Fujita KI, Ito M, Irie M, Harada K, Fujiwara N, Ikeda Y, Yoshioka H, Yamazaki T, Kojima M, Mikami B, Mayeda A, Masuda S Nat Commun. 2024 Jan 15;15(1):455. doi: 10.1038/s41467-023-44217-8. PMID:38225262[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pryor A, Tung L, Yang Z, Kapadia F, Chang TH, Johnson LF. Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56. Nucleic Acids Res. 2004 Mar 26;32(6):1857-65. PMID:15047853 doi:10.1093/nar/gkh347
- ↑ Sugiura T, Nagano Y, Noguchi Y. DDX39, upregulated in lung squamous cell cancer, displays RNA helicase activities and promotes cancer cell growth. Cancer Biol Ther. 2007 Jun;6(6):957-64. PMID:17548965 doi:10.4161/cbt.6.6.4192
- ↑ Fujita KI, Ito M, Irie M, Harada K, Fujiwara N, Ikeda Y, Yoshioka H, Yamazaki T, Kojima M, Mikami B, Mayeda A, Masuda S. Structural differences between the closely related RNA helicases, UAP56 and URH49, fashion distinct functional apo-complexes. Nat Commun. 2024 Jan 15;15(1):455. PMID:38225262 doi:10.1038/s41467-023-44217-8
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