8j8k

Structural highlights

Function

DPPRS_MYCTU Involved in the biosynthesis of decaprenylphosphoryl arabinose (DPA) a precursor for arabinan synthesis in mycobacterial cell wall biosynthesis (PubMed:15878857, PubMed:18310020). Catalyzes the transfer of a 5-phosphoribosyl residue from phosphoribose diphosphate (PRPP) to decaprenyl phosphate (DP) to form decaprenylphosphoryl-5-phosphoribose (DPPR) (PubMed:15878857, PubMed:18310020, PubMed:38491273). The enzyme favors polyprenyl phosphate with 50-60 carbon atoms, is unable to use C-20 polyprenyl phosphate, and uses C-75 polyprenyl phosphate less efficiently than C-50 or C-60 (PubMed:15878857). Cannot use UDP-galactose or GDP-mannose as a sugar donor (PubMed:15878857). May play a role in ethambutol (EMB) resistance (PubMed:25547657).^{[1] [2] [3] [4]}

References

1. ↑ Huang H, Scherman MS, D'Haeze W, Vereecke D, Holsters M, Crick DC, McNeil MR. Identification and active expression of the Mycobacterium tuberculosis gene encoding 5-phospho-{alpha}-d-ribose-1-diphosphate: decaprenyl-phosphate 5-phosphoribosyltransferase, the first enzyme committed to decaprenylphosphoryl-d-arabinose synthesis. J Biol Chem. 2005 Jul 1;280(26):24539-43. PMID:15878857 doi:10.1074/jbc.M504068200
2. ↑ Huang H, Berg S, Spencer JS, Vereecke D, D'Haeze W, Holsters M, McNeil MR. Identification of amino acids and domains required for catalytic activity of DPPR synthase, a cell wall biosynthetic enzyme of Mycobacterium tuberculosis. Microbiology (Reading). 2008 Mar;154(Pt 3):736-743. PMID:18310020 doi:10.1099/mic.0.2007/013532-0
3. ↑ He L, Wang X, Cui P, Jin J, Chen J, Zhang W, Zhang Y. ubiA (Rv3806c) encoding DPPR synthase involved in cell wall synthesis is associated with ethambutol resistance in Mycobacterium tuberculosis. Tuberculosis (Edinb). 2015 Mar;95(2):149-54. PMID:25547657 doi:10.1016/j.tube.2014.12.002
4. ↑ Gao S, Wu F, Gurcha SS, Batt SM, Besra GS, Rao Z, Zhang L. Structural analysis of phosphoribosyltransferase-mediated cell wall precursor synthesis in Mycobacterium tuberculosis. Nat Microbiol. 2024 Apr;9(4):976-987. PMID:38491273 doi:10.1038/s41564-024-01643-8