8k9y

Structural highlights

Function

SOT16_ARATH Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of desulfo-glucosinolates (dsGSs), the final step in the biosynthesis of the glucosinolate core structure. Substrate preference is desulfo-2-phenylethyl glucosinolate > desulfo-indol-3-yl methyl glucosinolate > desulfo-benzyl glucosinolate > desulfo-6-methylthiohexyl glucosinolate > desulfo-4-methylthiobutyl glucosinolate > desulfo-3-methylthiopropyl glucosinolate > desulfo-singrin > desulfo-3-butenyl glucosinolate.^{[1] [2] [3] [4] [5]}

See Also

• Sulfotransferase 3D structures

References

1. ↑ Piotrowski M, Schemenewitz A, Lopukhina A, Muller A, Janowitz T, Weiler EW, Oecking C. Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure. J Biol Chem. 2004 Dec 3;279(49):50717-25. Epub 2004 Sep 9. PMID:15358770 doi:http://dx.doi.org/10.1074/jbc.M407681200
2. ↑ Hirai MY, Klein M, Fujikawa Y, Yano M, Goodenowe DB, Yamazaki Y, Kanaya S, Nakamura Y, Kitayama M, Suzuki H, Sakurai N, Shibata D, Tokuhisa J, Reichelt M, Gershenzon J, Papenbrock J, Saito K. Elucidation of gene-to-gene and metabolite-to-gene networks in arabidopsis by integration of metabolomics and transcriptomics. J Biol Chem. 2005 Jul 8;280(27):25590-5. Epub 2005 May 2. PMID:15866872 doi:http://dx.doi.org/M502332200
3. ↑ Klein M, Reichelt M, Gershenzon J, Papenbrock J. The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed. FEBS J. 2006 Jan;273(1):122-36. PMID:16367753 doi:http://dx.doi.org/10.1111/j.1742-4658.2005.05048.x
4. ↑ Klein M, Papenbrock J. Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana. Physiol Plant. 2009 Feb;135(2):140-9. doi: 10.1111/j.1399-3054.2008.01182.x. Epub, 2008 Dec 5. PMID:19077143 doi:http://dx.doi.org/10.1111/j.1399-3054.2008.01182.x
5. ↑ Moldrup ME, Geu-Flores F, Olsen CE, Halkier BA. Modulation of sulfur metabolism enables efficient glucosinolate engineering. BMC Biotechnol. 2011 Jan 31;11:12. doi: 10.1186/1472-6750-11-12. PMID:21281472 doi:http://dx.doi.org/10.1186/1472-6750-11-12