8q6v

Structural highlights

Function

DXO_YEAST Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (By similarity). The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (By similarity). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs (PubMed:12897126, PubMed:20802481, PubMed:26101253). Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (PubMed:12897126, PubMed:20802481). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (PubMed:20802481). Stimulates exoribonuclease activity of RAT1, allowing it to degrade RNAs with stable secondary structure more effectively (PubMed:20802481). Required for the processing of nuclear mRNA and rRNA precursors (PubMed:10805743, PubMed:12612077, PubMed:16131592). May promote termination of transcription by RNA polymerase II (PubMed:15565157).[UniProtKB:O13836][UniProtKB:O70348]^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Xue Y, Bai X, Lee I, Kallstrom G, Ho J, Brown J, Stevens A, Johnson AW. Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol Cell Biol. 2000 Jun;20(11):4006-15. PMID:10805743 doi:10.1128/MCB.20.11.4006-4015.2000
2. ↑ Sydorskyy Y, Dilworth DJ, Yi EC, Goodlett DR, Wozniak RW, Aitchison JD. Intersection of the Kap123p-mediated nuclear import and ribosome export pathways. Mol Cell Biol. 2003 Mar;23(6):2042-54. PMID:12612077 doi:10.1128/MCB.23.6.2042-2054.2003
3. ↑ Das B, Butler JS, Sherman F. Degradation of normal mRNA in the nucleus of Saccharomyces cerevisiae. Mol Cell Biol. 2003 Aug;23(16):5502-15. PMID:12897126
4. ↑ Kim M, Krogan NJ, Vasiljeva L, Rando OJ, Nedea E, Greenblatt JF, Buratowski S. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature. 2004 Nov 25;432(7016):517-22. PMID:15565157 doi:http://dx.doi.org/nature03041
5. ↑ Fang F, Phillips S, Butler JS. Rat1p and Rai1p function with the nuclear exosome in the processing and degradation of rRNA precursors. RNA. 2005 Oct;11(10):1571-8. PMID:16131592 doi:10.1261/rna.2900205
6. ↑ Jiao X, Xiang S, Oh C, Martin CE, Tong L, Kiledjian M. Identification of a quality-control mechanism for mRNA 5'-end capping. Nature. 2010 Sep 30;467(7315):608-11. PMID:20802481 doi:10.1038/nature09338
7. ↑ Wang VY, Jiao X, Kiledjian M, Tong L. Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes. Nucleic Acids Res. 2015 Jun 22. pii: gkv620. PMID:26101253 doi:http://dx.doi.org/10.1093/nar/gkv620