8qtn

Structural highlights

Function

LAG1_YEAST Component of the ceramide synthase complex that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward hexacosanoyl-CoA (C26:0-CoA) (PubMed:11694577, PubMed:12869556). N-acylates sphinganine and phytosphingosine bases to form dihydroceramides and phytoceramides, respectively (PubMed:12869556). Redundant with LAC1. Facilitates ER-to-Golgi transport of GPI-anchored proteins. Involved in the aging process. Deletion of LAG1 results in a pronounced increase (approximately 50%) in mean and in maximum life span.^{[1] [2] [3] [4] [5] [6]}

References

1. ↑ Barz WP, Walter P. Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins. Mol Biol Cell. 1999 Apr;10(4):1043-59. PMID:10198056 doi:10.1091/mbc.10.4.1043
2. ↑ Guillas I, Kirchman PA, Chuard R, Pfefferli M, Jiang JC, Jazwinski SM, Conzelmann A. C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p. EMBO J. 2001 Jun 1;20(11):2655-65. PMID:11387200 doi:10.1093/emboj/20.11.2655
3. ↑ Schorling S, Vallée B, Barz WP, Riezman H, Oesterhelt D. Lag1p and Lac1p are essential for the Acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae. Mol Biol Cell. 2001 Nov;12(11):3417-27. PMID:11694577 doi:10.1091/mbc.12.11.3417
4. ↑ Guillas I, Jiang JC, Vionnet C, Roubaty C, Uldry D, Chuard R, Wang J, Jazwinski SM, Conzelmann A. Human homologues of LAG1 reconstitute Acyl-CoA-dependent ceramide synthesis in yeast. J Biol Chem. 2003 Sep 26;278(39):37083-91. PMID:12869556 doi:10.1074/jbc.M307554200
5. ↑ Jiang JC, Kirchman PA, Allen M, Jazwinski SM. Suppressor analysis points to the subtle role of the LAG1 ceramide synthase gene in determining yeast longevity. Exp Gerontol. 2004 Jul;39(7):999-1009. PMID:15236759 doi:10.1016/j.exger.2004.03.026
6. ↑ Vallée B, Riezman H. Lip1p: a novel subunit of acyl-CoA ceramide synthase. EMBO J. 2005 Feb 23;24(4):730-41. PMID:15692566 doi:10.1038/sj.emboj.7600562