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Publication Abstract from PubMed

Antascomicin B is a natural product that similarly to the macrolides FK506 and Rapamycin binds to the FK506-binding protein 12 (FKBP12). FK506 and Rapamycin act as molecular glues by inducing ternary complexes between FKBPs and additional target proteins. Whether Antascomicin B can induce ternary complexes is unknown. Here we show that Antascomicin B binds tightly to larger human FKBP homologs. The cocrystal structure of FKBP51 in complex with Antascomicin B revealed that large parts of Antascomicin B are solvent-exposed and available to engage additional proteins. Cellular studies demonstrated that Antascomicin B enhances the interaction between human FKBP51 and human Akt. Our studies show that molecules with molecular glue-like properties are more prominent in nature than previously thought. We predict the existence of additional 'orphan' molecular glues that evolved to induce ternary protein complexes but where the relevant ternary complex partners are unknown.

Antascomicin B stabilizes FKBP51-Akt1 complexes as a molecular glue.,Schafer SC, Voll AM, Bracher A, Ley SV, Hausch F Bioorg Med Chem Lett. 2024 May 15;104:129728. doi: 10.1016/j.bmcl.2024.129728. , Epub 2024 Apr 4. PMID:38582133^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.