8rs4

Structural highlights

Function

TRP6H_STRAO Involved in the biosynthesis of thienodolin, a plant growth-regulating compound (PubMed:24692213). Catalyzes the chlorination of tryptophan (Trp) at C6 position to yield 6-chloro-tryptophan (PubMed:24692213, PubMed:33465708). It is also able to use bromide ions to generate monobrominated Trp (PubMed:33465708, PubMed:36259362). In vitro, accepts a wide range of amides and peptides carrying either L- or D-Trp at the N-terminus (PubMed:36259362).^{[1] [2] [3]}

References

1. ↑ Milbredt D, Patallo EP, van Pée KH. A tryptophan 6-halogenase and an amidotransferase are involved in thienodolin biosynthesis. Chembiochem. 2014 May 5;15(7):1011-20. PMID:24692213 doi:10.1002/cbic.201400016
2. ↑ Phintha A, Prakinee K, Jaruwat A, Lawan N, Visitsatthawong S, Kantiwiriyawanitch C, Songsungthong W, Trisrivirat D, Chenprakhon P, Mulholland A, van Pée KH, Chitnumsub P, Chaiyen P. Dissecting the low catalytic capability of flavin-dependent halogenases. J Biol Chem. 2021 Jan-Jun;296:100068. PMID:33465708 doi:10.1074/jbc.RA120.016004
3. ↑ Schnepel C, Moritzer AC, Gafe S, Montua N, Minges H, Niess A, Niemann HH, Sewald N. Enzymatic Late-Stage Halogenation of Peptides. Chembiochem. 2022 Oct 19. doi: 10.1002/cbic.202200569. PMID:36259362 doi:http://dx.doi.org/10.1002/cbic.202200569