8s3l

Structural highlights

Function

LP9A_PANSI Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 or C4 oxidation product (PubMed:26928935, PubMed:29057953, PubMed:32818374). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (PubMed:26928935, PubMed:29057953). Is able to cleave phosphoric acid swollen cellulose (PASC) in the presence of a reducing agent, yielding a range of cellooligosaccharides dominated by cellobiose and cellotriose (PubMed:26928935). Activity is less sensitive to the reducing agent potential when cleaving xylan, suggesting that distinct catalytic mechanisms exist for xylan and glucan cleavage (PubMed:29057953).^{[1] [2] [3]}

References

1. ↑ Frandsen KE, Simmons TJ, Dupree P, Poulsen JN, Hemsworth GR, Ciano L, Johnston EM, Tovborg M, Johansen KS, von Freiesleben P, Marmuse L, Fort S, Cottaz S, Driguez H, Henrissat B, Lenfant N, Tuna F, Baldansuren A, Davies GJ, Lo Leggio L, Walton PH. The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases. Nat Chem Biol. 2016 Feb 29. doi: 10.1038/nchembio.2029. PMID:26928935 doi:http://dx.doi.org/10.1038/nchembio.2029
2. ↑ Simmons TJ, Frandsen KEH, Ciano L, Tryfona T, Lenfant N, Poulsen JC, Wilson LFL, Tandrup T, Tovborg M, Schnorr K, Johansen KS, Henrissat B, Walton PH, Lo Leggio L, Dupree P. Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates. Nat Commun. 2017 Oct 20;8(1):1064. doi: 10.1038/s41467-017-01247-3. PMID:29057953 doi:http://dx.doi.org/10.1038/s41467-017-01247-3
3. ↑ Tandrup T, Tryfona T, Frandsen KEH, Johansen KS, Dupree P, Lo Leggio L. Oligosaccharide Binding and Thermostability of Two Related AA9 Lytic Polysaccharide Monooxygenases. Biochemistry. 2020 Sep 15;59(36):3347-3358. doi: 10.1021/acs.biochem.0c00312., Epub 2020 Aug 27. PMID:32818374 doi:http://dx.doi.org/10.1021/acs.biochem.0c00312