| Structural highlights
Function
TIL_ARATH Involved in basal (BT) and acquired thermotolerance (AT), probably by preventing plasma membrane lipids peroxidation induced by severe heat-shock (HS) (PubMed:19302169, PubMed:23837879). Lipocalin that confers protection against oxidative stress caused by heat, freezing, paraquat and light (PubMed:18671872, PubMed:19302169). Confers resistance to high salt (NaCl) levels, probably by protecting chloroplasts from ion toxicity via ion homeostasis maintenance (PubMed:20959419, PubMed:24028869). Required for seed longevity by ensuring polyunsaturated lipids integrity (PubMed:23837879).[1] [2] [3] [4] [5]
Publication Abstract from PubMed
Arabidopsis thaliana temperature-induced lipocalin (AtTIL) is a prototypical member of plant lipocalins and participates in a variety of cellular processes, particularly stress responses. Bioinformatical and physiological studies have proposed its promiscuous ligand-binding ability, but the molecular basis is yet unclear. Here, we report the 1.9-A crystal structure of AtTIL in complex with heme. Spectrophotometric absorbance titration with heme yields a dissociation constant of approximately 2 micromolar, indicating the relatively weak interaction between AtTIL and heme, which is confirmed by the AtTIL-heme structure. Although binding to retinal or biliverdin is not detected, such possibility can not be precluded as suggested by comparison with other lipocalin structures. These results show that AtTIL is a structural and functional homolog of the bacterial lipocalin Blc.
Crystallographic and functional studies of a plant temperature-induced lipocalin.,Dong CS, Zhang WL, Wang XY, Wang X, Wang J, Wang M, Fang Y, Liu L Biochim Biophys Acta Gen Subj. 2023 Dec 14;1868(2):130540. doi: , 10.1016/j.bbagen.2023.130540. PMID:38103756[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Charron JB, Ouellet F, Houde M, Sarhan F. The plant Apolipoprotein D ortholog protects Arabidopsis against oxidative stress. BMC Plant Biol. 2008 Jul 31;8:86. PMID:18671872 doi:10.1186/1471-2229-8-86
- ↑ Chi WT, Fung RW, Liu HC, Hsu CC, Charng YY. Temperature-induced lipocalin is required for basal and acquired thermotolerance in Arabidopsis. Plant Cell Environ. 2009 Jul;32(7):917-27. PMID:19302169 doi:10.1111/j.1365-3040.2009.01972.x
- ↑ Brinker M, Brosché M, Vinocur B, Abo-Ogiala A, Fayyaz P, Janz D, Ottow EA, Cullmann AD, Saborowski J, Kangasjärvi J, Altman A, Polle A. Linking the salt transcriptome with physiological responses of a salt-resistant Populus species as a strategy to identify genes important for stress acclimation. Plant Physiol. 2010 Dec;154(4):1697-709. PMID:20959419 doi:10.1104/pp.110.164152
- ↑ Boca S, Koestler F, Ksas B, Chevalier A, Leymarie J, Fekete A, Mueller MJ, Havaux M. Arabidopsis lipocalins AtCHL and AtTIL have distinct but overlapping functions essential for lipid protection and seed longevity. Plant Cell Environ. 2014 Feb;37(2):368-81. PMID:23837879 doi:10.1111/pce.12159
- ↑ Abo-Ogiala A, Carsjens C, Diekmann H, Fayyaz P, Herrfurth C, Feussner I, Polle A. Temperature-induced lipocalin (TIL) is translocated under salt stress and protects chloroplasts from ion toxicity. J Plant Physiol. 2014 Feb 15;171(3-4):250-9. PMID:24028869 doi:10.1016/j.jplph.2013.08.003
- ↑ Dong CS, Zhang WL, Wang XY, Wang X, Wang J, Wang M, Fang Y, Liu L. Crystallographic and functional studies of a plant temperature-induced lipocalin. Biochim Biophys Acta Gen Subj. 2023 Dec 14;1868(2):130540. PMID:38103756 doi:10.1016/j.bbagen.2023.130540
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