| Structural highlights
Function
CSCL2_MOUSE Mechanosensitive cation channel with low conductance and high activation threshold. Essential for the baby's first breath and respiration throughout life. Upon lung inflation conducts cation currents in alveolar type 1 and 2 cells triggering lamellar body exocytosis and surfactant secretion into airspace (PubMed:30382938, PubMed:37543036, PubMed:38127458). Osmosensitive cation channel preferentially activated by hypotonic stress. Acts as an osmosensor in cochlear outer hair cells (OHCs) where it mediates calcium influx and regulatory volume decrease response. Required for the maintenance of OHC morphology, OHC survival and normal hearing (PubMed:27045885, PubMed:31243992, PubMed:32375046, PubMed:37543036, PubMed:37945568).[1] [2] [3] [4] [5] [6] [7] GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
References
- ↑ Zhao X, Yan X, Liu Y, Zhang P, Ni X. Co-expression of mouse TMEM63A, TMEM63B and TMEM63C confers hyperosmolarity activated ion currents in HEK293 cells. Cell Biochem Funct. 2016 Jun;34(4):238-41. PMID:27045885 doi:10.1002/cbf.3185
- ↑ Murthy SE, Dubin AE, Whitwam T, Jojoa-Cruz S, Cahalan SM, Mousavi SAR, Ward AB, Patapoutian A. OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels. Elife. 2018 Nov 1;7:e41844. PMID:30382938 doi:10.7554/eLife.41844
- ↑ Marques MC, Albuquerque IS, Vaz SH, Bernardes GJL. Overexpression of Osmosensitive Ca(2+)-Permeable Channel TMEM63B Promotes Migration in HEK293T Cells. Biochemistry. 2019 Jul 2;58(26):2861-2866. PMID:31243992 doi:10.1021/acs.biochem.9b00224
- ↑ Du H, Ye C, Wu D, Zang YY, Zhang L, Chen C, He XY, Yang JJ, Hu P, Xu Z, Wan G, Shi YS. The Cation Channel TMEM63B Is an Osmosensor Required for Hearing. Cell Rep. 2020 May 5;31(5):107596. PMID:32375046 doi:10.1016/j.celrep.2020.107596
- ↑ Zheng W, Rawson S, Shen Z, Tamilselvan E, Smith HE, Halford J, Shen C, Murthy SE, Ulbrich MH, Sotomayor M, Fu TM, Holt JR. TMEM63 proteins function as monomeric high-threshold mechanosensitive ion channels. Neuron. 2023 Jul 28:S0896-6273(23)00512-3. PMID:37543036 doi:10.1016/j.neuron.2023.07.006
- ↑ Qin Y, Yu D, Wu D, Dong J, Li WT, Ye C, Cheung KC, Zhang Y, Xu Y, Wang Y, Shi YS, Dang S. Cryo-EM structure of TMEM63C suggests it functions as a monomer. Nat Commun. 2023 Nov 9;14(1):7265. PMID:37945568 doi:10.1038/s41467-023-42956-2
- ↑ Chen GL, Li JY, Chen X, Liu JW, Zhang Q, Liu JY, Wen J, Wang N, Lei M, Wei JP, Yi L, Li JJ, Ling YP, Yi HQ, Hu Z, Duan J, Zhang J, Zeng B. Mechanosensitive channels TMEM63A and TMEM63B mediate lung inflation-induced surfactant secretion. J Clin Invest. 2024 Dec 21;134(5):e174508. PMID:38127458 doi:10.1172/JCI174508
|
