8x6m

Structural highlights

Function

GLDA_ECOLI Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. Possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively.^{[1] [2] [3] [4]}

References

1. ↑ Subedi KP, Kim I, Kim J, Min B, Park C. Role of GldA in dihydroxyacetone and methylglyoxal metabolism of Escherichia coli K12. FEMS Microbiol Lett. 2008 Feb;279(2):180-7. doi:, 10.1111/j.1574-6968.2007.01032.x. Epub 2007 Dec 20. PMID:18179582 doi:http://dx.doi.org/10.1111/j.1574-6968.2007.01032.x
2. ↑ Gonzalez R, Murarka A, Dharmadi Y, Yazdani SS. A new model for the anaerobic fermentation of glycerol in enteric bacteria: trunk and auxiliary pathways in Escherichia coli. Metab Eng. 2008 Sep;10(5):234-45. Epub 2008 May 27. PMID:18632294 doi:http://dx.doi.org/S1096-7176(08)00020-7
3. ↑ Tang CT, Ruch FE Jr, Lin CC. Purification and properties of a nicotinamide adenine dinucleotide-linked dehydrogenase that serves an Escherichia coli mutant for glycerol catabolism. J Bacteriol. 1979 Oct;140(1):182-7. PMID:40950
4. ↑ Truniger V, Boos W. Mapping and cloning of gldA, the structural gene of the Escherichia coli glycerol dehydrogenase. J Bacteriol. 1994 Mar;176(6):1796-800. PMID:8132480