8xag

Structural highlights

Function

LHP1_ARATH Structural component of heterochromatin involved in gene repression, including several floral homeotic genes and FLT that regulates flowering time. Required for maintenance of vernalization-induced repression of FLC. As part of the PRC1-like complex, recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me), leading to epigenetic repression. PcG PRC1 complex maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Transcriptional repressor that binds DNA on GAGA-like motif and 5'-(A/G/T)AACCCTA(A/G)-3' consensus motif in the promoters of target genes. Recognizes and binds histone H3 tails methylated at 'Lys-27' (H3K27me) but depleted in active histone marks such as H3K4me, leading to epigenetic repression. When in complex with LHP1, recognizes and binds histone H3 tails methylated at 'Lys-4' (H3K4me) and 'Lys-27' (H3K27me), mostly corresponding to stress-responsive genes (PubMed:27495811).^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Gaudin V, Libault M, Pouteau S, Juul T, Zhao G, Lefebvre D, Grandjean O. Mutations in LIKE HETEROCHROMATIN PROTEIN 1 affect flowering time and plant architecture in Arabidopsis. Development. 2001 Dec;128(23):4847-58. doi: 10.1242/dev.128.23.4847. PMID:11731464 doi:http://dx.doi.org/10.1242/dev.128.23.4847
2. ↑ Kotake T, Takada S, Nakahigashi K, Ohto M, Goto K. Arabidopsis TERMINAL FLOWER 2 gene encodes a heterochromatin protein 1 homolog and represses both FLOWERING LOCUS T to regulate flowering time and several floral homeotic genes. Plant Cell Physiol. 2003 Jun;44(6):555-64. doi: 10.1093/pcp/pcg091. PMID:12826620 doi:http://dx.doi.org/10.1093/pcp/pcg091
3. ↑ Kim JH, Durrett TP, Last RL, Jander G. Characterization of the Arabidopsis TU8 glucosinolate mutation, an allele of TERMINAL FLOWER2. Plant Mol Biol. 2004 Mar;54(5):671-82. PMID:15356387 doi:http://dx.doi.org/10.1023/B:PLAN.0000040897.49151.98
4. ↑ Mylne JS, Barrett L, Tessadori F, Mesnage S, Johnson L, Bernatavichute YV, Jacobsen SE, Fransz P, Dean C. LHP1, the Arabidopsis homologue of HETEROCHROMATIN PROTEIN1, is required for epigenetic silencing of FLC. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5012-7. Epub 2006 Mar 20. PMID:16549797 doi:http://dx.doi.org/0507427103
5. ↑ Sung S, He Y, Eshoo TW, Tamada Y, Johnson L, Nakahigashi K, Goto K, Jacobsen SE, Amasino RM. Epigenetic maintenance of the vernalized state in Arabidopsis thaliana requires LIKE HETEROCHROMATIN PROTEIN 1. Nat Genet. 2006 Jun;38(6):706-10. doi: 10.1038/ng1795. Epub 2006 May 7. PMID:16682972 doi:http://dx.doi.org/10.1038/ng1795
6. ↑ Xu L, Shen WH. Polycomb silencing of KNOX genes confines shoot stem cell niches in Arabidopsis. Curr Biol. 2008 Dec 23;18(24):1966-71. doi: 10.1016/j.cub.2008.11.019. PMID:19097900 doi:http://dx.doi.org/10.1016/j.cub.2008.11.019
7. ↑ Molitor AM, Latrasse D, Zytnicki M, Andrey P, Houba-Herin N, Hachet M, Battail C, Del Prete S, Alberti A, Quesneville H, Gaudin V. The Arabidopsis hnRNP-Q Protein LIF2 and the PRC1 Subunit LHP1 Function in Concert to Regulate the Transcription of Stress-Responsive Genes. Plant Cell. 2016 Sep;28(9):2197-2211. doi: 10.1105/tpc.16.00244. Epub 2016 Aug 5. PMID:27495811 doi:http://dx.doi.org/10.1105/tpc.16.00244