Structural highlights
Function
ACT_BACMT Involved in the activation of the NAD-dependent methanol dehydrogenase (MDH). MDH activation by Act involves hydrolytic removal of the nicotinamide mononucleotide (NMN) moiety of the NAD cofactor, changing its ping-pong type of reaction mechanism into a ternary complex reaction mechanism. It requires the presence of magnesium ions and is also able to use ADP-ribose.[1] [2]
References
- ↑ Kloosterman H, Vrijbloed JW, Dijkhuizen L. Molecular, biochemical, and functional characterization of a Nudix hydrolase protein that stimulates the activity of a nicotinoprotein alcohol dehydrogenase. J Biol Chem. 2002 Sep 20;277(38):34785-92. PMID:12089158 doi:10.1074/jbc.M205617200
- ↑ Arfman N, Van Beeumen J, De Vries GE, Harder W, Dijkhuizen L. Purification and characterization of an activator protein for methanol dehydrogenase from thermotolerant Bacillus spp. J Biol Chem. 1991 Feb 25;266(6):3955-60 PMID:1995643
