9env

Structural highlights

Function

BLAG1_KLEPN Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, second, third and fourth-generation cephalosporins (PubMed:10681329, PubMed:12936982, PubMed:19656947, PubMed:20696873, PubMed:29507065). Has ceftazidime-hydrolyzing activity (PubMed:10681329, PubMed:12936982, PubMed:19656947, PubMed:20696873, PubMed:29507065). Inactive against the carbapenems, imipenem, meropenem, ertapenem and doripenem (PubMed:10681329, PubMed:19656947, PubMed:29507065). However, weak hydrolytic activity with respect to imipenem has also been reported (PubMed:20696873).^{[1] [2] [3] [4] [5]}

References

1. ↑ Poirel L, Le Thomas I, Naas T, Karim A, Nordmann P. Biochemical sequence analyses of GES-1, a novel class A extended-spectrum beta-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2000 Mar;44(3):622-32. PMID:10681329 doi:10.1128/AAC.44.3.622-632.2000
2. ↑ Correia M, Boavida F, Grosso F, Salgado MJ, Lito LM, Cristino JM, Mendo S, Duarte A. Molecular characterization of a new class 3 integron in Klebsiella pneumoniae. Antimicrob Agents Chemother. 2003 Sep;47(9):2838-43. PMID:12936982 doi:10.1128/AAC.47.9.2838-2843.2003
3. ↑ Frase H, Shi Q, Testero SA, Mobashery S, Vakulenko SB. Mechanistic basis for the emergence of catalytic competence against carbapenem antibiotics by the GES family of beta-lactamases. J Biol Chem. 2009 Oct 23;284(43):29509-13. PMID:19656947 doi:10.1074/jbc.M109.011262
4. ↑ Kotsakis SD, Miriagou V, Tzelepi E, Tzouvelekis LS. Comparative biochemical and computational study of the role of naturally occurring mutations at Ambler positions 104 and 170 in GES β-lactamases. Antimicrob Agents Chemother. 2010 Nov;54(11):4864-71. PMID:20696873 doi:10.1128/AAC.00771-10
5. ↑ Piccirilli A, Mercuri PS, Galleni M, Aschi M, Matagne A, Amicosante G, Perilli M. P174E Substitution in GES-1 and GES-5 β-Lactamases Improves Catalytic Efficiency toward Carbapenems. Antimicrob Agents Chemother. 2018 Apr 26;62(5):e01851-17. PMID:29507065 doi:10.1128/AAC.01851-17