Structural highlights
9fq6 is a 11 chain structure with sequence from Vaccinia virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | Electron Microscopy, Resolution 2.5Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
RAP94_VACCC DNA-directed RNA polymerase-associated factor required for the transcription of viral early genes as well as for transcription termination. Within minutes after virus entry, recruits the core RNA polymerase, the early transcription factor (ETF) and other enzymes needed for transcription initiation, elongation, and termination thereby allowing synthesis of early mRNAs which are extruded through pores in the core particle. Recruits the multifunctional OPG102 protein, with poly(A) polymerase-stimulatory, cap nucleoside-2'-O-methyltransferase, and transcription elongation activities. Interacts with nucleoside triphosphatase I/OPG123, a DNA-dependent ATPase required for the termination of early transcripts. Acts as a transcription termination factor by binding, together with the capping enzyme/VTF, to the termination motif 5'-UUUUUNU-3' in the nascent mRNA. Involved as well in the packaging of RNA polymerase and other components needed for early transcription in assembling virus particles.[UniProtKB:P68438]
