Structural highlights
Function
IPA25_SHIFL E3 ubiquitin-protein ligase effector that inhibits host cell innate immunity during bacterial infection by catalyzing 'Lys-48'-linked polyubiquitination and subsequent degradation of host RNF31/HOIP (PubMed:27572974, PubMed:36610722). Host RNF31/HOIP is the catalytic component of the LUBAC complex, which conjugates linear ('Met-1'-linked) polyubiquitin chains at the surface of bacteria invading the host cytosol to form the ubiquitin coat surrounding bacteria (PubMed:27572974). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed:27572974).[1] [2]
References
- ↑ de Jong MF, Liu Z, Chen D, Alto NM. Shigella flexneri suppresses NF-κB activation by inhibiting linear ubiquitin chain ligation. Nat Microbiol. 2016 May 27;1(7):16084. PMID:27572974 doi:10.1038/nmicrobiol.2016.84
- ↑ Hiragi K, Nishide A, Takagi K, Iwai K, Kim M, Mizushima T. Structural insight into the recognition of the linear ubiquitin assembly complex by Shigella E3 ligase IpaH1.4/2.5. J Biochem. 2023 Mar 31;173(4):317-326. PMID:36610722 doi:10.1093/jb/mvac109
