Structural highlights
Function
NFD2_ARATH Required for karyogamy during female gametophyte development, when the two polar nuclei fuse to form the diploid central cell nucleus.[1]
Publication Abstract from PubMed
The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.
Protein crystallization and structure determination at room temperature in the CrystalChip.,Pachl P, Coudray L, Vincent R, Nilles L, Scheer H, Ritzenthaler C, Fejfarova A, Rezacova P, Engilberge S, Sauter C FEBS Open Bio. 2024 Nov 21. doi: 10.1002/2211-5463.13932. PMID:39572886[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Portereiko MF, Sandaklie-Nikolova L, Lloyd A, Dever CA, Otsuga D, Drews GN. NUCLEAR FUSION DEFECTIVE1 encodes the Arabidopsis RPL21M protein and is required for karyogamy during female gametophyte development and fertilization. Plant Physiol. 2006 Jul;141(3):957-65. doi: 10.1104/pp.106.079319. Epub 2006 May , 12. PMID:16698901 doi:http://dx.doi.org/10.1104/pp.106.079319
- ↑ Pachl P, Coudray L, Vincent R, Nilles L, Scheer H, Ritzenthaler C, Fejfarová A, Řezáčová P, Engilberge S, Sauter C. Protein crystallization and structure determination at room temperature in the CrystalChip. FEBS Open Bio. 2024 Nov 21. PMID:39572886 doi:10.1002/2211-5463.13932
