9hdd
From Proteopedia
Sla2 C-terminal region (Residues 560-968) (REND and THATCH domains)
Structural highlights
FunctionSLA2_YEAST Required for cellular morphogenesis and polarization of the cortical cytoskeleton. It might act in concert with proteins such as CDC42 and CDC43 to limit the region of cortical patch formation to the cortex of the bud. Required for the accumulation and/or maintenance of plasma membrane H(+)-ATPase on the cell surface. Publication Abstract from PubMedThe interaction network of Sla2, a vital endocytic mid-coat adaptor protein, undergoes constant rearrangement. Sla2 serves as a scaffold linking the membrane to the actin cytoskeleton, with its role modulated by the clathrin light chain (CLC), which inhibits Sla2's function under certain conditions. We show that Sla2 has two independent binding sites for CLC: one previously described in homologs of fungi (Sla2) and metazoa (Hip1R), and a second found only in Fungi. We present the structural model of the Sla2 actin-binding domains in the context of regulatory structural domains by cryoelectron microscopy. We provide an interaction map of Sla2 and the regulatory proteins Sla1 and Pan1, predicted by AI modeling and confirmed by molecular biophysics techniques. Pan1 may compete with CLC for the conserved Sla2-binding site. These results enhance the mapping of crucial interactions at endocytic checkpoints and highlight the divergence between Metazoa and Fungi in this vital process. Sla2 is a core interaction hub for clathrin light chain and the Pan1/End3/Sla1 complex.,Draper-Barr G, Defelipe LA, Ruiz-Carrillo D, Gustavsson E, Landau M, Garcia-Alai M Structure. 2025 Apr 28:S0969-2126(25)00147-9. doi: 10.1016/j.str.2025.04.013. PMID:40347949[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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