9k7i

Structural highlights

Function

GGP1_ARATH Involved in glucosinolate biosynthesis. Hydrolyzes the gamma-glutamyl peptide bond of several glutathione (GSH) conjugates to produce Cys-Gly conjugates related to glucosinolates. The gamma-Glu-Cys-Gly-GSH conjugates are the sulfur-donating molecule in glucosinolate biosynthesis (PubMed:19483696, PubMed:21712415). Converts phenylacetohydroximoyl-GSH to benzylglucosinolate (PubMed:19483696). Can use the GSH conjugate of the camalexin intermediate IAN (GS-IAN) as substrate. Required for the biosynthesis of camalexin, a pathogen-inducible phytoalexin with antibacterial and antifungal properties (PubMed:21712415).^{[1] [2]}

References

1. ↑ Geu-Flores F, Nielsen MT, Nafisi M, Møldrup ME, Olsen CE, Motawia MS, Halkier BA. Glucosinolate engineering identifies a gamma-glutamyl peptidase. Nat Chem Biol. 2009 Aug;5(8):575-7. PMID:19483696 doi:10.1038/nchembio.185
2. ↑ Geu-Flores F, Møldrup ME, Böttcher C, Olsen CE, Scheel D, Halkier BA. Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis. Plant Cell. 2011 Jun;23(6):2456-69. PMID:21712415 doi:10.1105/tpc.111.083998