Structural highlights
Publication Abstract from PubMed
The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition.
AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA.,Kim DY, Lee SY, Ha HJ, Park HH Proc Natl Acad Sci U S A. 2025 Apr 8;122(14):e2423205122. doi: , 10.1073/pnas.2423205122. Epub 2025 Apr 3. PMID:40178896[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim DY, Lee SY, Ha HJ, Park HH. AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA. Proc Natl Acad Sci U S A. 2025 Apr 8;122(14):e2423205122. PMID:40178896 doi:10.1073/pnas.2423205122
