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Publication Abstract from PubMed

Glycolytic enzymes sense metabolite levels to adapt rapidly to changing energy demands, but measuring the levels of these effectors with spatiotemporal precision in live cells has been challenging. We addressed this question in the context of neuronal depolarization, which activates glycolysis, focusing on the glycolysis inhibitor citrate. We engineered a pair of quantitative fluorescent biosensors for citrate that address several limitations (affinity, pH, Mg(2+), and temperature) of existing citrate biosensors. Using two-photon fluorescence lifetime imaging, we found that free citrate in the cytosol of neurons in acute mouse brain slices declines two-to-threefold within seconds of neuronal activation and then returns to baseline over a few minutes. The stimulation-dependent citrate transient depends at least in part on the mitochondrial calcium uniporter. These types of live metabolite measurements are essential for achieving a nuanced understanding of the fast control of glycolysis.

Activity-dependent citrate dynamics in neurons.,Rosen PC, Fu P, Ferran B, Kim E, Brooks DJ, Lim DC, Diaz-Garcia CM, Yellen G Proc Natl Acad Sci U S A. 2025 Oct 14;122(41):e2519902122. doi: , 10.1073/pnas.2519902122. Epub 2025 Oct 10. PMID:41071660^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.