9n3s

Structural highlights

Function

OCH1_YEAST Mannosyltransferase involved in outer chain elongation of asparagine-linked oligosaccharides of the type Man(9)GlcNAc(2). Adds the first alpha-1,6-mannose to the Man(8)GlcNAc(2) and Man(9)GlcNAc(2), but not Man(5)GlcNAc(2), endoplasmic reticulum intermediates. Represents the first enzymatic event required for synthesis of outer chain mannose linkages on yeast secretory proteins. Also has the potential to transfer a second alpha-1,6-mannose to the Man(8)GlcNAc(2) core oligosaccharide.^{[1] [2] [3] [4] [5] [6]}

References

1. ↑ Nagasu T, Shimma Y, Nakanishi Y, Kuromitsu J, Iwama K, Nakayama K, Suzuki K, Jigami Y. Isolation of new temperature-sensitive mutants of Saccharomyces cerevisiae deficient in mannose outer chain elongation. Yeast. 1992 Jul;8(7):535-47. PMID:1523886 doi:10.1002/yea.320080705
2. ↑ Nakayama K, Nagasu T, Shimma Y, Kuromitsu J, Jigami Y. OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharides. EMBO J. 1992 Jul;11(7):2511-9. PMID:1628616 doi:10.1002/j.1460-2075.1992.tb05316.x
3. ↑ Kitajima T, Chiba Y, Jigami Y. Saccharomyces cerevisiae alpha1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule. FEBS J. 2006 Nov;273(22):5074-85. PMID:17042779 doi:10.1111/j.1742-4658.2006.05505.x
4. ↑ Chantret I, Kodali VP, Lahmouich C, Harvey DJ, Moore SEH. Endoplasmic reticulum-associated degradation (ERAD) and free oligosaccharide generation in Saccharomyces cerevisiae. J Biol Chem. 2011 Dec 2;286(48):41786-41800. PMID:21979948 doi:10.1074/jbc.M111.251371
5. ↑ Lehle L, Eiden A, Lehnert K, Haselbeck A, Kopetzki E. Glycoprotein biosynthesis in Saccharomyces cerevisiae: ngd29, an N-glycosylation mutant allelic to och1 having a defect in the initiation of outer chain formation. FEBS Lett. 1995 Aug 14;370(1-2):41-5. PMID:7649302 doi:10.1016/0014-5793(95)00789-c
6. ↑ Nakanishi-Shindo Y, Nakayama K, Tanaka A, Toda Y, Jigami Y. Structure of the N-linked oligosaccharides that show the complete loss of alpha-1,6-polymannose outer chain from och1, och1 mnn1, and och1 mnn1 alg3 mutants of Saccharomyces cerevisiae. J Biol Chem. 1993 Dec 15;268(35):26338-45 PMID:8253757