Structural highlights
Function
GOGA7_HUMAN May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.[1] [2]
Publication Abstract from PubMed
Small molecules serve as valuable tools for probing nonapoptotic cell death mechanisms. The small molecule caspase independent lethal 56 (CIL56) induces a unique form of nonapoptotic cancer cell death that is promoted by a complex formed between zDHHC palmitoyltransferase 5 (ZDHHC5) and an accessory protein, golgin A7 (GOLGA7, also known as GCP16). The structure and function of this complex in nonapoptotic cell death regulation remain poorly understood. Here, we use coimmunoprecipitation, functional assays, and cryogenic electron microscopy (cryo-EM) to elucidate the structure and function of the Zdhhc5-GOLGA7 complex. We identify key residues in both Zdhhc5 and GOLGA7 that are necessary for complex formation and to promote nonapoptotic cancer cell death in response to caspase independent lethal 56. These results provide new insights into the structure and function of a death-promoting protein complex.
Functional dissection of the zDHHC palmitoyltransferase 5-golgin A7 palmitoylation complex.,Kahlson MA, Ritho J, Gomes JV, Wang H, Butterwick JA, Dixon SJ J Biol Chem. 2025 Sep 8;301(10):110694. doi: 10.1016/j.jbc.2025.110694. PMID:40930250[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ohta E, Misumi Y, Sohda M, Fujiwara T, Yano A, Ikehara Y. Identification and characterization of GCP16, a novel acylated Golgi protein that interacts with GCP170. J Biol Chem. 2003 Dec 19;278(51):51957-67. PMID:14522980 doi:10.1074/jbc.M310014200
- ↑ Swarthout JT, Lobo S, Farh L, Croke MR, Greentree WK, Deschenes RJ, Linder ME. DHHC9 and GCP16 constitute a human protein fatty acyltransferase with specificity J Biol Chem. 2005 Sep 2;280(35):31141-8. PMID:16000296 doi:10.1074/jbc.M504113200
- ↑ Kahlson MA, Ritho J, Gomes JV, Wang H, Butterwick JA, Dixon SJ. Functional dissection of the zDHHC palmitoyltransferase 5-golgin A7 palmitoylation complex. J Biol Chem. 2025 Sep 8;301(10):110694. PMID:40930250 doi:10.1016/j.jbc.2025.110694
