9vkf

Structural highlights

Function

ANM1_YEAST S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that catalyzes both the mono- and asymmetric (type I) dimethylation of the guanidino nitrogens of arginine residues in a variety of RNA-binding proteins such as heterogeneous nuclear ribonucleoproteins (hnRNPs) and small nuclear ribonucleoproteins (snRNPs) (PubMed:8647869, PubMed:8668183). Methylates NAB2, NPL3, HRP1 and YRA1, shuttling hnRNPs involved in mRNA processing and export, facilitating their export out of the nucleus (PubMed:10652296, PubMed:11779864, PubMed:15314027, PubMed:8668183, PubMed:9499403). Methylation of NPL3 weakens its interaction with THO2, a component of the TREX (transcription/export) complex important for transcriptional elongation and recruitment of mRNA export factors (PubMed:15314027, PubMed:20053728). Methylates the hnRNP HRB1, but does not influence its subcellular location (PubMed:9499403). Methylates the nucleolar proteins GAR1, NOP1 and NSR1 (PubMed:12756332). Methylates the snRNP SNP1 and modulates the cotranscriptional recruitment of splicing factors (PubMed:20823272). Dimethylates free histone H4 (HHF1/HHF2) at 'Arg-4' (H4R3me2a) and plays a role in preservation and establishment of silent chromatin domains (PubMed:12097318, PubMed:17158743). Mono- and dimethylates ribosomal protein S2 (RPS2) at 'Arg-11' (PubMed:20035717). Methylates the catalytic subunit of the SWI/SNF chromatin-remodeling complex SNF2 (PubMed:22997150).^{[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14]}

References

1. ↑ McBride AE, Weiss VH, Kim HK, Hogle JM, Silver PA. Analysis of the yeast arginine methyltransferase Hmt1p/Rmt1p and its in vivo function. Cofactor binding and substrate interactions. J Biol Chem. 2000 Feb 4;275(5):3128-36. PMID:10652296 doi:10.1074/jbc.275.5.3128
2. ↑ Weiss VH, McBride AE, Soriano MA, Filman DJ, Silver PA, Hogle JM. The structure and oligomerization of the yeast arginine methyltransferase, Hmt1. Nat Struct Biol. 2000 Dec;7(12):1165-71. PMID:11101900 doi:10.1038/82028
3. ↑ Green DM, Marfatia KA, Crafton EB, Zhang X, Cheng X, Corbett AH. Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p. J Biol Chem. 2002 Mar 8;277(10):7752-60. PMID:11779864 doi:10.1074/jbc.M110053200
4. ↑ Lacoste N, Utley RT, Hunter JM, Poirier GG, Cote J. Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase. J Biol Chem. 2002 Aug 23;277(34):30421-4. Epub 2002 Jul 3. PMID:12097318 doi:http://dx.doi.org/10.1074/jbc.C200366200
5. ↑ Xu C, Henry PA, Setya A, Henry MF. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. RNA. 2003 Jun;9(6):746-59. PMID:12756332 doi:10.1261/rna.5020803
6. ↑ Yu MC, Bachand F, McBride AE, Komili S, Casolari JM, Silver PA. Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors. Genes Dev. 2004 Aug 15;18(16):2024-35. PMID:15314027 doi:10.1101/gad.1223204
7. ↑ Yu MC, Lamming DW, Eskin JA, Sinclair DA, Silver PA. The role of protein arginine methylation in the formation of silent chromatin. Genes Dev. 2006 Dec 1;20(23):3249-54. PMID:17158743 doi:10.1101/gad.1495206
8. ↑ Lipson RS, Webb KJ, Clarke SG. Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2010 Jan 22;391(4):1658-62. PMID:20035717 doi:10.1016/j.bbrc.2009.12.112
9. ↑ Wong CM, Tang HM, Kong KY, Wong GW, Qiu H, Jin DY, Hinnebusch AG. Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p. Nucleic Acids Res. 2010 Apr;38(7):2217-28. PMID:20053728 doi:10.1093/nar/gkp1133
10. ↑ Chen YC, Milliman EJ, Goulet I, Côté J, Jackson CA, Vollbracht JA, Yu MC. Protein arginine methylation facilitates cotranscriptional recruitment of pre-mRNA splicing factors. Mol Cell Biol. 2010 Nov;30(21):5245-56. PMID:20823272 doi:10.1128/MCB.00359-10
11. ↑ Jackson CA, Yadav N, Min S, Li J, Milliman EJ, Qu J, Chen YC, Yu MC. Proteomic analysis of interactors for yeast protein arginine methyltransferase Hmt1 reveals novel substrate and insights into additional biological roles. Proteomics. 2012 Nov;12(22):3304-14. PMID:22997150 doi:10.1002/pmic.201200132
12. ↑ Gary JD, Lin WJ, Yang MC, Herschman HR, Clarke S. The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae. J Biol Chem. 1996 May 24;271(21):12585-94. PMID:8647869 doi:10.1074/jbc.271.21.12585
13. ↑ Henry MF, Silver PA. A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins. Mol Cell Biol. 1996 Jul;16(7):3668-78. PMID:8668183 doi:10.1128/MCB.16.7.3668
14. ↑ Shen EC, Henry MF, Weiss VH, Valentini SR, Silver PA, Lee MS. Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev. 1998 Mar 1;12(5):679-91. PMID:9499403