Enolase multiple sequence alignment

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This page is a supplement to Introduction to Evolutionary Conservation. The portion of the multiple sequence alignment (MSA) below with  a pink background  is enlarged on that page.

Multiple Sequence Alignment (MSA) for enolase, an enzyme in glycolysis.

Key: IDENTICAL(*)   SIMILAR(.)   different

Consensus Percentage is set to 100%.

 1    ENO_DROME| Drosophila melanogaster  --------mt IkaIkARqIy DSRGNPTVEv DLtt-elglf rAavPSGAST 41
 2              ENOA_HUMAN| Homo sapiens  ---------s IlkIhAReIf DSRGNPTVEv DLft-skglf rAavPSGAST 40
 3    ENO_METJA| Methanococcus janaschii  mlynmderfe IkdIvAReVi DSRGNPTVEv EVitkgngyg sAivPSGAST 50
 4           ENO_ECOLI| Escherichia coli  --------sk IvkIiGReIi DSRGNPTVEa EVhleggfvg mAaaPSGAST 42
 5          ENO_BACSU| Bacillus subtilis  -------mpy IvdVyAReVl DSRGNPTVEv EVytetgafg rAlvPSGAST 43
 6  4ENL Yeast| Saccharomyces cerevisiae  ---------a VskVyARsVy DSRGNPTVEv ELtt-ekgvf rSivPSGAST 40

                                                     .  . .* .  *********  ..          .  ******

 1    ENO_DROME| Drosophila melanogaster  GvhEALElRD nDKanYhGKS VlkAVghVNd tLgpelIkan ldvvdQasID 91
 2              ENOA_HUMAN| Homo sapiens  GiyEALElRD nDKtrYmGKG VskAVehINk tIapalVskk lnvteQekID 90
 3    ENO_METJA| Methanococcus janaschii  GthEALElRD kEK-rFgGKG VlmAVenVNs iIrpeiLg-- ydarmQreID 97
 4           ENO_ECOLI| Escherichia coli  GsrEALElRD gDKsrFlGKG VtkAVaaVNg pIaqalIg-- kdakdQagID 90
 5          ENO_BACSU| Bacillus subtilis  GeyEAVElRD gDKdrYlGKG VltAVnnVNe iIapelLg-- fdvteQnaID 91
 6  4ENL Yeast| Saccharomyces cerevisiae  GvhEALEmRD gDKskWmGKG VlhAVknVNd vIapafVkan idvsdQkaVD 90

                                          *  **.* **  .*  . **. *  **  .*   .    .         *  .*

 1    ENO_DROME| Drosophila melanogaster  nfmIklDGTe NKSKfGANAI LGVSlAvaKA GAakkgvPLY khIAdlagn- 140
 2              ENOA_HUMAN| Homo sapiens  klmIemDGTe NKSKfGANAI LGVSlAvcKA GAvekgvPLY rhIAdlagn- 139
 3    ENO_METJA| Methanococcus janaschii  timIelDGTp NKSRlGANAI LAVSlAvaKA AAatakiPLY kyLGgfn--- 144
 4           ENO_ECOLI| Escherichia coli  kimIdlDGTe NKSKfGANAI LAVSlAnaKA AAaakgmPLY ehIAelngt- 139
 5          ENO_BACSU| Bacillus subtilis  qllIelDGTe NKGKlGANAI LGVSmAcaRA AAdflqiPLY qyLGgfn--- 138
 6  4ENL Yeast| Saccharomyces cerevisiae  dflIslDGTa NKSKlGANAI LGVSlAasRA AAaeknvPLY khLAdlsksk 140

                                             *  ***  **.. ***** *.** *  .* .*     ***    .

 1    ENO_DROME| Drosophila melanogaster  -keiilPVPa fNVINGGsHA gnkLamQEFM IlPtGAtsft EAmKmGSeVY 189
 2              ENOA_HUMAN| Homo sapiens  -sevilPVPa fNVINGGsHA gnkLamQEFM IlPvGAanfr EAmRiGAeVY 188
 3    ENO_METJA| Methanococcus janaschii  --syvmPVPm mNVINGGkHA gndLdlQEFM ImPvGAtsis EAvRmGSeVY 192
 4           ENO_ECOLI| Escherichia coli  pgkysmPVPm mNIINGGeHA dnnVdiQEFM IqPvGAktvk EAiRmGSeVF 189
 5          ENO_BACSU| Bacillus subtilis  --sktlPVPm mNIVNGGeHA dnnVdiQEFM ImPvGApnfr EAlRmGAqIF 186
 6  4ENL Yeast| Saccharomyces cerevisiae  tspyvlPVPf lNVLNGGsHA ggaLalQEFM IaPtGAktfa EAlRiGSeVY 190

                                                ***   *..*** **    .  **** * * **     ** . *. ..

 1    ENO_DROME| Drosophila melanogaster  HhLknVikaK fgldataVGD EGGfAPnIqs nkEALnlIsd AIakAGyt-- 237
 2              ENOA_HUMAN| Homo sapiens  HnLknVikeK ygkdatnVGD EGGfAPnIle nkEGLelLkt AIgkAGyt-- 236
 3    ENO_METJA| Methanococcus janaschii  HvLknVileK ygknavnVGD EGGfAPpLkt srEALdlLte SVkkAGye-- 240
 4           ENO_ECOLI| Escherichia coli  HhLakVlkaK g--mntaVGD EGGyAPnLgs naEALavIae AVkaAGyelg 237
 5          ENO_BACSU| Bacillus subtilis  HsLksVlsaK g--lntaVGD EGGfAPnLgs neEALqtIve AIekAGfkpg 234
 6  4ENL Yeast| Saccharomyces cerevisiae  HnLksLtkkR ygasagnVGD EGGvAPnIqt aeEALdlIvd AIkaAG---- 238

                                          **  .   .        *** *** ** .     *.*  .   ..  **
                                         
 1    ENO_DROME| Drosophila melanogaster  gkIeiGmDvA ASEFYk--dG qYdLdfknek sdksqwlpad klanlykefi 285
 2              ENOA_HUMAN| Homo sapiens  dkVviGmDvA ASEFFr--sG kYdLdfksp- ddpsryispd qladlyksfi 283
 3    ENO_METJA| Methanococcus janaschii  deVvfAlDaA ASEFYk--dG yYyVegkk-- ------ltre elldyykalv 280
 4           ENO_ECOLI| Escherichia coli  kdItlAmDcA ASEFYk--dG kYvLagegn- ----kaftse efthfleelt 280
 5          ENO_BACSU| Bacillus subtilis  eeVklAmDaA SSEFYnkedG kYhLsgeg-- ----vvktsa emvdwyeelv 278
 6  4ENL Yeast| Saccharomyces cerevisiae  gkVkiGlDcA SSEFFk--dG kYdLdfknpn sdkskwltgp qladlyhslm 286

                                            .  . * * .***.    *  * .                 
                                         
 1    ENO_DROME| Drosophila melanogaster  kdFPIVSIED pfdqdhWeAw snltgctd-- IQIVGDDLtV TNpkrIatAV 333
 2              ENOA_HUMAN| Homo sapiens  kdYPVVSIED pfdqddWgAw qkftasag-- IQVVGDDLtV TNpkrIakAV 331
 3    ENO_METJA| Methanococcus janaschii  deYPIVSIED pfheedFeGf amitkeld-- IQIVGDDLfV TNverLrkGI 328
 4           ENO_ECOLI| Escherichia coli  kqYPIVSIED gldesdWdGf ayqtkvlgdk IQLVGDDLfV TNtkiLkeGI 330
 5          ENO_BACSU| Bacillus subtilis  skYPIISIED gldendWeGh kllterlgkk VQLVGDDLfV TNtkkLseGI 328
 6  4ENL Yeast| Saccharomyces cerevisiae  krYPIVSIED pfaeddWeAw shffktag-- IQIVADDLtV TNpkrIatAI 334

                                            .*..****       . .             .*.*.*** * **   .  ..

 1    ENO_DROME| Drosophila melanogaster  ekkacncLLL KvNQIGtVtE siaAhllAkk nGWgtmVSHR SGETEDsfIG 383
 2              ENOA_HUMAN| Homo sapiens  nekscncLLL KvNQIGsVtE slqAcklAqa nGWgvmVSHR SGETEDtfIA 381
 3    ENO_METJA| Methanococcus janaschii  emkaanaLLL KvNQIGtLsE avdAaqlAfr nGYgvvVSHR SGETEDttIA 378
 4           ENO_ECOLI| Escherichia coli  ekgiansILI KfNQIGsLtE tlaAikmAkd aGYtavISHR SGETEDatIA 380
 5          ENO_BACSU| Bacillus subtilis  kngvgnsILI KvNQIGtLtE tfdAiemAkr aGYtavISHR SGETEDstIA 378
 6  4ENL Yeast| Saccharomyces cerevisiae  ekkaadaLLL KvNQIGtLsE sikAaqdSfa aGWgvmVSHR SGETEDtfIA 384

                                                 .*. . **** . *    *   .    *.   .*** ******  *.

 1    ENO_DROME| Drosophila melanogaster  DLvVGlstGQ IKTGApcRsE RlAKyNQILR IEeeIGagvk FaGksfgkpq 433
 2              ENOA_HUMAN| Homo sapiens  DLvVGlctGQ IKTGApcRsE RlAKyNQLLR IEeeLGskak FaGrnfrnpl 431
 3    ENO_METJA| Methanococcus janaschii  DLsVAlnsGQ IKTGApaRgE RtAKyNQLIR IEqeLG-lsk YaGrnfrcpf 427
 4           ENO_ECOLI| Escherichia coli  DLaVGtaaGQ IKTGSmsRsD RvAKyNQLIR IEeaLGekap YnGrkeikgq 430
 5          ENO_BACSU| Bacillus subtilis  DIaVAtnaGQ IKTGApsRtD RvAKyNQLLR IEdqLAetaq YhGinsfynl 428
 6  4ENL Yeast| Saccharomyces cerevisiae  DLvVGlrtGQ IKTGApaRsE RlAKlNQLLR IEeeLGdnav FaGenfhhgd 434

                                          *. *.   ** ****.  * . * ** **..* **  ..     . *

 1    ENO_DROME| Drosophila melanogaster  -- 433
 2              ENOA_HUMAN| Homo sapiens  ak 433
 3    ENO_METJA| Methanococcus janaschii  -- 427
 4           ENO_ECOLI| Escherichia coli  a- 431
 5          ENO_BACSU| Bacillus subtilis  nk 430
 6  4ENL Yeast| Saccharomyces cerevisiae  kl 436

Summary of categories applied to a total of 436 residues in the PDB file sequence for a consensus level setting of 100%:

33% (142) IDENTICAL(*) 16% (68) SIMILAR(.) 52% (226) different

Definitions of SIMILAR groups of amino acids:
ILV   Ile Leu Val      hydrophobic, aliphatic / medium-sized side-chain
FWY   Phe Trp Tyr      hydrophobic, aromatic / large side-chain (Tyr polar)
KRH   Lys Arg His      + charge
DE    Asp Glu          - charge
GAS   Gly Ala Ser      small
P     Pro              helix breaking
C     Cys              disulfide-forming
TNQM  Thr Asn Gln Met  polar (except Met)

Proteopedia Page Contributors and Editors (what is this?)

Eric Martz

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