Crystal structure of a hypothetical protein from Giardia lamblia
Dylan Beard, Seonna Bristol, Kayla Cosby, Amber Davis, Courtney Manning, Lionel Perry, Lauren Snapp, Arian Toy, Kayla Wheeler, Jeremy Young, Bart L Staker, David Dranow, Jan Abendroth, Sandhya Subramanian, Thomas E Edwards, Peter J Myler, and Oluwatoyin A. Asojo [1]
Molecular Tour
Giardiasis or traveler's diarrhea can be fatal to the young, elderly, or immunocompromised. The Seattle Structural Genomics Center for Infectious Disease (SSGCID) has determined the structures of potential drug targets from Giardia lamblia. Some of these proteins are hypothetical proteins that share < 30% sequence identity or less to proteins with known structure (see static image below).
Despite the low sequence similarity the hypothetical protein is a prototypical YjgF/YER057c/UK114 endoribonuclease
The atomic resolution structure of a 15.6 kDa protein was determined by molecular replacement (PDB entry 3i3f). The structure has observed in the prototypical endoribonucleases L-PSP with conserved allosteric active sites containing small molecules from the crystallization solution. Alpha Helices,
310 Helices,
Pi Helices,
Beta Strands ,
Loops ,
Turns. . Chains A, B, and C of GilaA.00312.a trimer are colored in green, cyan, and magenta, respectively. The closest structures are colored in gray. These studies are part of ongoing studies to identify proteins that could be targeted for rational drug discovery for giardiasis. This paper is an educational collaboration between Hampton University (an HBCU) and the Seattle Structural Genomics Center for Infectious Disease (SSGCID).
PDB reference: hypothetical protein from Giardia lamblia, 3i3f.
References
- ↑ Beard DK, Bristol S, Cosby K, Davis A, Manning C, Perry L, Snapp L, Toy A, Wheeler K, Young J, Staker B, Arakaki TL, Abendroth J, Subrahamanian S, Edwards TE, Myler PJ, Asojo OA. Crystal structure of a hypothetical protein from Giardia lamblia. Acta Crystallogr F Struct Biol Commun. 2022 Feb 1;78(Pt 2):59-65. doi:, 10.1107/S2053230X21013595. Epub 2022 Jan 28. PMID:35102894 doi:http://dx.doi.org/10.1107/S2053230X21013595
