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The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project'

This shows the basic structure of the kinase domain with an . The ATP will sit between them just below Beta 1

shows the ATP binding site (purple) and the catalytic loop (green).

Here it is as a .

These are found in other RTKs.

G1000S (magenta), A1133G (orange), T1221I (teal)

And as a

This when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster

Using the software TS_pred (a ts mutation prediction program): these are the to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red)

Using Rosetta_ts_rbf (another ts mutation prediction program): these are the sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow)

Both TS_pred and Rosetta_ts_rbf had in their top 5 (black)

Most but not all of the actual and predicted mutations are . Note that T1221 and G1000S are not buried.

Here are the .