Function
Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.
- Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands[1].
- Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt).
- Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.
- RadA recombinase promotes DNA recombination. Detailed analysis of its suggests mechanisms for junction isomerization and communication between the two active sites [2].
- RadB recombinase is a paralogue of RadA assisting in RadA-mediated strand exchange [3].
- Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions[4].
- Tre recombinase recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells [5].
3D structures of resolvase
Resolvase 3D structures
