Rhomboid protease

From Proteopedia

Function Rhomboid protease (GlpG) is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain[1]. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins. Rhomboid protease YqgP interacts with the membrane bound metlloprotease FisH and cleaves the high-affinity Mg transporter[2]. Structural highlights The inhibitor isocoumarin covalently binds to the GlpG catalytic residues Ser and His (in deepskyblue)[3]. Whole active site. Water molecules shown as red spheres.

spinqualitylabelsall models Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry 2xow) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of rhomboid protease

Updated on 08-August-2024

Rhomboid protease GlpG

2ic8, 2irv, 2nrf, 2o7l, 3b45, 2xov, 4njn, 4njp – EcGlpG core region – Escherichia coli

2xtu, 3b44, 2xtv, 5f5d – EcGlpG core region (mutant)

4hdd – EcGlpG N terminal

2lep, 2mja – EcGlpG N terminal - NMR

3odj – HiGlpG - Haemophilus influenzae

2nr9 – HiGlpG (mutant) + detergent

2gqc – RP N terminal – NMR – Pseudomonas aeruginosa

Rhomboid protease GlpG complex with inhibitor

2xow, 3zeb – EcGlpG core region + isocoumarin inhibitor

3txt, 4h1d – EcGlpG core region + DFP inhibitor

3ubb – EcGlpG core region + phosphonofluoridate inhibitor

3zmh, 3zmi, 3zmj, 3zot – EcGlpG core region + β-lactam inhibitor

6pj5, 6pj7, 6pj8, 6pj9, 6pja, 6pjp, 6pjq, 6pjr, 6pju – EcGlpG core region + peptide aldehyde inhibitor

5f5j, 5f5k, 6pj4 – EcGlpG core region (mutant) + peptide aldehyde inhibitor

4qo0, 4qo2, 5f5g, 5f5b, 5mtf, 5mt6, 5mt7, 5mt8, 6vj8, 6jv9, 6vjp, 6xro, 6xrp – EcGlpG core region + peptide inhibitor

4qnz – EcGlpG core region (mutant) + peptide inhibitor

Rhomboid protease YqgP

6r0j – YqpG – Bacillus subtilis - NMR

Rhomboid protease GluP

7tj4 – GluP + LytH – Staphylococcus aureus

References

1. ↑ Freeman M. Rhomboid proteases and their biological functions. Annu Rev Genet. 2008;42:191-210. doi: 10.1146/annurev.genet.42.110807.091628. PMID:18605900 doi:http://dx.doi.org/10.1146/annurev.genet.42.110807.091628
2. ↑ Began J, Cordier B, Brezinova J, Delisle J, Hexnerova R, Srb P, Rampirova P, Kozisek M, Baudet M, Coute Y, Galinier A, Veverka V, Doan T, Strisovsky K. Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease. EMBO J. 2020 Jan 13:e102935. doi: 10.15252/embj.2019102935. PMID:31930742 doi:http://dx.doi.org/10.15252/embj.2019102935
3. ↑ Vinothkumar KR, Strisovsky K, Andreeva A, Christova Y, Verhelst S, Freeman M. The structural basis for catalysis and substrate specificity of a rhomboid protease. EMBO J. 2010 Oct 1. PMID:20890268 doi:10.1038/emboj.2010.243